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Biochemical properties of GH94 cellodextrin phosphorylase THA_1941 from a thermophilic eubacterium Thermosipho africanus TCF52B with cellobiose phosphorylase activity
A hypothetic gene (THA_1941) encoding a putative cellobiose phosphorylase (CBP) from Thermosipho africanus TCF52B has very low amino acid identities (less than 12%) to all known GH94 enzymes. This gene was cloned and over-expressed in Escherichia coli BL21(DE3). The recombinant protein was hypothesi...
Autores principales: | Wu, Yuanyuan, Mao, Guotao, Fan, Haiyan, Song, Andong, Zhang, Yi-Heng Percival, Chen, Hongge |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5501786/ https://www.ncbi.nlm.nih.gov/pubmed/28687766 http://dx.doi.org/10.1038/s41598-017-05289-x |
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