Inactivation of Pol θ and C-NHEJ eliminates off-target integration of exogenous DNA

Off-target or random integration of exogenous DNA hampers precise genomic engineering and presents a safety risk in clinical gene therapy strategies. Genetic definition of random integration has been lacking for decades. Here, we show that the A-family DNA polymerase θ (Pol θ) promotes random integr...

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Detalles Bibliográficos
Autores principales: Zelensky, Alex N., Schimmel, Joost, Kool, Hanneke, Kanaar, Roland, Tijsterman, Marcel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5501794/
https://www.ncbi.nlm.nih.gov/pubmed/28687761
http://dx.doi.org/10.1038/s41467-017-00124-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5501794/
https://www.ncbi.nlm.nih.gov/pubmed/28687761
http://dx.doi.org/10.1038/s41467-017-00124-3

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