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Streamlined Membrane Proteome Preparation for Shotgun Proteomics Analysis with Triton X-100 Cloud Point Extraction and Nanodiamond Solid Phase Extraction

While mass spectrometry (MS) plays a key role in proteomics research, characterization of membrane proteins (MP) by MS has been a challenging task because of the presence of a host of interfering chemicals in the hydrophobic protein extraction process, and the low protease digestion efficiency. We r...

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Detalles Bibliográficos
Autores principales: Pham, Minh D., Wen, Ting-Chun, Li, Hung-Cheng, Hsieh, Pei-Hsuan, Chen, Yet-Ran, Chang, Huan-Cheng, Han, Chau-Chung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5503057/
https://www.ncbi.nlm.nih.gov/pubmed/28773508
http://dx.doi.org/10.3390/ma9050385