Cargando…

Presence of diabetes autoantigens in extracellular vesicles derived from human islets

Beta-cell (β-cell) injury is the hallmark of autoimmune diabetes. However, the mechanisms by which autoreactive responses are generated in susceptible individuals are not well understood. Extracellular vesicles (EV) are produced by mammalian cells under normal and stressed physiological states. They...

Descripción completa

Detalles Bibliográficos
Autores principales: Hasilo, Craig P., Negi, Sarita, Allaeys, Isabelle, Cloutier, Nathalie, Rutman, Alissa K., Gasparrini, Marco, Bonneil, Éric, Thibault, Pierre, Boilard, Éric, Paraskevas, Steven
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5504025/
https://www.ncbi.nlm.nih.gov/pubmed/28694505
http://dx.doi.org/10.1038/s41598-017-04977-y
_version_ 1783249201940922368
author Hasilo, Craig P.
Negi, Sarita
Allaeys, Isabelle
Cloutier, Nathalie
Rutman, Alissa K.
Gasparrini, Marco
Bonneil, Éric
Thibault, Pierre
Boilard, Éric
Paraskevas, Steven
author_facet Hasilo, Craig P.
Negi, Sarita
Allaeys, Isabelle
Cloutier, Nathalie
Rutman, Alissa K.
Gasparrini, Marco
Bonneil, Éric
Thibault, Pierre
Boilard, Éric
Paraskevas, Steven
author_sort Hasilo, Craig P.
collection PubMed
description Beta-cell (β-cell) injury is the hallmark of autoimmune diabetes. However, the mechanisms by which autoreactive responses are generated in susceptible individuals are not well understood. Extracellular vesicles (EV) are produced by mammalian cells under normal and stressed physiological states. They are an important part of cellular communication, and may serve a role in antigen processing and presentation. We hypothesized that isolated human islets in culture produce EV that contain diabetes autoantigens (DAA) from these otherwise normal, non-diabetic donors. Here we report the caspase-independent production of EV by human islets in culture, and the characterization of DAA glutamic acid decarboxylase 65 (GAD65) and zinc transporter 8 (ZnT8), as well as the β-cell resident glucose transporter 2 (Glut2), present within the EV.
format Online
Article
Text
id pubmed-5504025
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-55040252017-07-12 Presence of diabetes autoantigens in extracellular vesicles derived from human islets Hasilo, Craig P. Negi, Sarita Allaeys, Isabelle Cloutier, Nathalie Rutman, Alissa K. Gasparrini, Marco Bonneil, Éric Thibault, Pierre Boilard, Éric Paraskevas, Steven Sci Rep Article Beta-cell (β-cell) injury is the hallmark of autoimmune diabetes. However, the mechanisms by which autoreactive responses are generated in susceptible individuals are not well understood. Extracellular vesicles (EV) are produced by mammalian cells under normal and stressed physiological states. They are an important part of cellular communication, and may serve a role in antigen processing and presentation. We hypothesized that isolated human islets in culture produce EV that contain diabetes autoantigens (DAA) from these otherwise normal, non-diabetic donors. Here we report the caspase-independent production of EV by human islets in culture, and the characterization of DAA glutamic acid decarboxylase 65 (GAD65) and zinc transporter 8 (ZnT8), as well as the β-cell resident glucose transporter 2 (Glut2), present within the EV. Nature Publishing Group UK 2017-07-10 /pmc/articles/PMC5504025/ /pubmed/28694505 http://dx.doi.org/10.1038/s41598-017-04977-y Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Hasilo, Craig P.
Negi, Sarita
Allaeys, Isabelle
Cloutier, Nathalie
Rutman, Alissa K.
Gasparrini, Marco
Bonneil, Éric
Thibault, Pierre
Boilard, Éric
Paraskevas, Steven
Presence of diabetes autoantigens in extracellular vesicles derived from human islets
title Presence of diabetes autoantigens in extracellular vesicles derived from human islets
title_full Presence of diabetes autoantigens in extracellular vesicles derived from human islets
title_fullStr Presence of diabetes autoantigens in extracellular vesicles derived from human islets
title_full_unstemmed Presence of diabetes autoantigens in extracellular vesicles derived from human islets
title_short Presence of diabetes autoantigens in extracellular vesicles derived from human islets
title_sort presence of diabetes autoantigens in extracellular vesicles derived from human islets
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5504025/
https://www.ncbi.nlm.nih.gov/pubmed/28694505
http://dx.doi.org/10.1038/s41598-017-04977-y
work_keys_str_mv AT hasilocraigp presenceofdiabetesautoantigensinextracellularvesiclesderivedfromhumanislets
AT negisarita presenceofdiabetesautoantigensinextracellularvesiclesderivedfromhumanislets
AT allaeysisabelle presenceofdiabetesautoantigensinextracellularvesiclesderivedfromhumanislets
AT cloutiernathalie presenceofdiabetesautoantigensinextracellularvesiclesderivedfromhumanislets
AT rutmanalissak presenceofdiabetesautoantigensinextracellularvesiclesderivedfromhumanislets
AT gasparrinimarco presenceofdiabetesautoantigensinextracellularvesiclesderivedfromhumanislets
AT bonneileric presenceofdiabetesautoantigensinextracellularvesiclesderivedfromhumanislets
AT thibaultpierre presenceofdiabetesautoantigensinextracellularvesiclesderivedfromhumanislets
AT boilarderic presenceofdiabetesautoantigensinextracellularvesiclesderivedfromhumanislets
AT paraskevassteven presenceofdiabetesautoantigensinextracellularvesiclesderivedfromhumanislets