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Isolation of membrane vesicles from prokaryotes: a technical and biological comparison reveals heterogeneity

Prokaryotes release membrane vesicles (MVs) with direct roles in disease pathogenesis. MVs are heterogeneous when isolated from bacterial cultures so Density Gradient Centrifugation (DGC) is valuable for separation of MV subgroups from contaminating material. Here we report the technical variability...

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Detalles Bibliográficos
Autores principales: Dauros Singorenko, Priscila, Chang, Vanessa, Whitcombe, Alana, Simonov, Denis, Hong, Jiwon, Phillips, Anthony, Swift, Simon, Blenkiron, Cherie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5505020/
https://www.ncbi.nlm.nih.gov/pubmed/28717421
http://dx.doi.org/10.1080/20013078.2017.1324731