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Isolation of membrane vesicles from prokaryotes: a technical and biological comparison reveals heterogeneity
Prokaryotes release membrane vesicles (MVs) with direct roles in disease pathogenesis. MVs are heterogeneous when isolated from bacterial cultures so Density Gradient Centrifugation (DGC) is valuable for separation of MV subgroups from contaminating material. Here we report the technical variability...
Autores principales: | Dauros Singorenko, Priscila, Chang, Vanessa, Whitcombe, Alana, Simonov, Denis, Hong, Jiwon, Phillips, Anthony, Swift, Simon, Blenkiron, Cherie |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5505020/ https://www.ncbi.nlm.nih.gov/pubmed/28717421 http://dx.doi.org/10.1080/20013078.2017.1324731 |
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