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An intragenic mutagenesis strategy in Physcomitrella patens to preserve intron splicing

Gene targeting is a powerful reverse genetics technique for site-specific genome modification. Intrinsic homologous recombination in the moss Physcomitrella patens permits highly effective gene targeting, a characteristic that makes this organism a valuable model for functional genetics. Functional...

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Detalles Bibliográficos
Autores principales: Ako, Ako Eugene, Perroud, Pierre-François, Innocent, Joseph, Demko, Viktor, Olsen, Odd-Arne, Johansen, Wenche
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5505980/
https://www.ncbi.nlm.nih.gov/pubmed/28698618
http://dx.doi.org/10.1038/s41598-017-05309-w