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An intragenic mutagenesis strategy in Physcomitrella patens to preserve intron splicing
Gene targeting is a powerful reverse genetics technique for site-specific genome modification. Intrinsic homologous recombination in the moss Physcomitrella patens permits highly effective gene targeting, a characteristic that makes this organism a valuable model for functional genetics. Functional...
Autores principales: | Ako, Ako Eugene, Perroud, Pierre-François, Innocent, Joseph, Demko, Viktor, Olsen, Odd-Arne, Johansen, Wenche |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5505980/ https://www.ncbi.nlm.nih.gov/pubmed/28698618 http://dx.doi.org/10.1038/s41598-017-05309-w |
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