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Comparisons of ELISA and Western blot assays for detection of autophagy flux

We analyzed autophagy/mitophagy flux in vitro (C2C12 myotubes) and in vivo (mouse skeletal muscle) following the treatments of autophagy inducers (starvation, rapamycin) and a mitophagy inducer (carbonyl cyanide m-chlorophenylhydrazone, CCCP) using two immunodetection methods, ELISA and Western blot...

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Detalles Bibliográficos
Autores principales: Oh, Sung-hee, Choi, Yong-bok, Kim, June-hyun, Weihl, Conrad C., Ju, Jeong-sun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5506881/
https://www.ncbi.nlm.nih.gov/pubmed/28725675
http://dx.doi.org/10.1016/j.dib.2017.06.045