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Comparisons of ELISA and Western blot assays for detection of autophagy flux
We analyzed autophagy/mitophagy flux in vitro (C2C12 myotubes) and in vivo (mouse skeletal muscle) following the treatments of autophagy inducers (starvation, rapamycin) and a mitophagy inducer (carbonyl cyanide m-chlorophenylhydrazone, CCCP) using two immunodetection methods, ELISA and Western blot...
Autores principales: | Oh, Sung-hee, Choi, Yong-bok, Kim, June-hyun, Weihl, Conrad C., Ju, Jeong-sun |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5506881/ https://www.ncbi.nlm.nih.gov/pubmed/28725675 http://dx.doi.org/10.1016/j.dib.2017.06.045 |
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