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FTIR spectroscopy of whole cells for the monitoring of yeast apoptosis mediated by p53 over-expression and its suppression by Nigella sativa extracts

p53 over expression in yeast results in cell death with typical markers of apoptosis such as DNA fragmentation and phosphatidylserine externalization. We aimed to substitute/supplement classical fluorescent techniques (TUNEL, Annexin V, ROS detection) usually used to detect biochemical changes occur...

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Detalles Bibliográficos
Autores principales: Mihoubi, Wafa, Sahli, Emna, Gargouri, Ali, Amiel, Caroline
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5507515/
https://www.ncbi.nlm.nih.gov/pubmed/28704406
http://dx.doi.org/10.1371/journal.pone.0180680