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15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell

BACKGROUND: A great number of studies reported that 12/15-lipoxygenase (12/15-LO) played an important role in atherosclerosis. And its arachidonic acid(AA) metabolite, 15(S)-hydroperoxy-5,8,11,13-(Z,Z,Z,E)-eicosatetraenoic acid (15(S)-HETE), is demonstrated to mediate endothelial dysfunction. 15-oxo...

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Autores principales: Ma, Guohua, Pan, Bing, Ren, Sufen, Guo, Caixia, Guo, Yansong, Wei, Lixin, Zheng, Lemin, Chen, Buxing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5508789/
https://www.ncbi.nlm.nih.gov/pubmed/28701173
http://dx.doi.org/10.1186/s12944-017-0518-2
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author Ma, Guohua
Pan, Bing
Ren, Sufen
Guo, Caixia
Guo, Yansong
Wei, Lixin
Zheng, Lemin
Chen, Buxing
author_facet Ma, Guohua
Pan, Bing
Ren, Sufen
Guo, Caixia
Guo, Yansong
Wei, Lixin
Zheng, Lemin
Chen, Buxing
author_sort Ma, Guohua
collection PubMed
description BACKGROUND: A great number of studies reported that 12/15-lipoxygenase (12/15-LO) played an important role in atherosclerosis. And its arachidonic acid(AA) metabolite, 15(S)-hydroperoxy-5,8,11,13-(Z,Z,Z,E)-eicosatetraenoic acid (15(S)-HETE), is demonstrated to mediate endothelial dysfunction. 15-oxo-5,8,11,13-(Z,Z,Z,E)-eicosatetraenoic acid (15-oxo-ETE) was formed from 15-hydroxyprostaglandin dehydrogenase (PGDH)-mediated oxidation of 15(S)-HETE. However, relatively little is known about the biological effects of 15-oxo-ETE in cardiovascular disease. Here, we explore the likely role of 15-lipoxygenase (LO)-1-mediated AA metabolism,15-oxo-ETE, in the early pathogenesis of atherosclerosis. METHODS: The 15-oxo-ETE level in serum was detected by means of liquid chromatography and online tandem mass spectrometry (LC-MS/MS). And the underlying mechanisms were illuminated by molecular techniques, including immunoblotting, MTT assay, immunocytochemistry and Immunohistochemistry. RESULTS: Increased 15-oxo-ETE level is found in in patients with acute myocardial infarction (AMI). After 15-oxo-ETE treatment, Human umbilical vein endothelial cells (HUVECs) showed more attractive to monocytes, whereas monocyte adhesion is suppressed when treated with PKC inhibitor. In ex vivo study, exposure of arteries from C57 mice and ApoE−/−mice to 15-oxo-ETE led to significantly increased E-selectin expression and monocyte adhesion. CONCLUSIONS: This is the first report that 15-oxo-ETE promotes early pathological process of atherosclerosis by accelerating E-selectin expression and monocyte adhesion. 15-oxo-ETE -induced monocyte adhesion is partly attributable to activation of PKC. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12944-017-0518-2) contains supplementary material, which is available to authorized users.
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spelling pubmed-55087892017-07-17 15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell Ma, Guohua Pan, Bing Ren, Sufen Guo, Caixia Guo, Yansong Wei, Lixin Zheng, Lemin Chen, Buxing Lipids Health Dis Research BACKGROUND: A great number of studies reported that 12/15-lipoxygenase (12/15-LO) played an important role in atherosclerosis. And its arachidonic acid(AA) metabolite, 15(S)-hydroperoxy-5,8,11,13-(Z,Z,Z,E)-eicosatetraenoic acid (15(S)-HETE), is demonstrated to mediate endothelial dysfunction. 15-oxo-5,8,11,13-(Z,Z,Z,E)-eicosatetraenoic acid (15-oxo-ETE) was formed from 15-hydroxyprostaglandin dehydrogenase (PGDH)-mediated oxidation of 15(S)-HETE. However, relatively little is known about the biological effects of 15-oxo-ETE in cardiovascular disease. Here, we explore the likely role of 15-lipoxygenase (LO)-1-mediated AA metabolism,15-oxo-ETE, in the early pathogenesis of atherosclerosis. METHODS: The 15-oxo-ETE level in serum was detected by means of liquid chromatography and online tandem mass spectrometry (LC-MS/MS). And the underlying mechanisms were illuminated by molecular techniques, including immunoblotting, MTT assay, immunocytochemistry and Immunohistochemistry. RESULTS: Increased 15-oxo-ETE level is found in in patients with acute myocardial infarction (AMI). After 15-oxo-ETE treatment, Human umbilical vein endothelial cells (HUVECs) showed more attractive to monocytes, whereas monocyte adhesion is suppressed when treated with PKC inhibitor. In ex vivo study, exposure of arteries from C57 mice and ApoE−/−mice to 15-oxo-ETE led to significantly increased E-selectin expression and monocyte adhesion. CONCLUSIONS: This is the first report that 15-oxo-ETE promotes early pathological process of atherosclerosis by accelerating E-selectin expression and monocyte adhesion. 15-oxo-ETE -induced monocyte adhesion is partly attributable to activation of PKC. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12944-017-0518-2) contains supplementary material, which is available to authorized users. BioMed Central 2017-07-12 /pmc/articles/PMC5508789/ /pubmed/28701173 http://dx.doi.org/10.1186/s12944-017-0518-2 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Ma, Guohua
Pan, Bing
Ren, Sufen
Guo, Caixia
Guo, Yansong
Wei, Lixin
Zheng, Lemin
Chen, Buxing
15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell
title 15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell
title_full 15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell
title_fullStr 15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell
title_full_unstemmed 15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell
title_short 15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell
title_sort 15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5508789/
https://www.ncbi.nlm.nih.gov/pubmed/28701173
http://dx.doi.org/10.1186/s12944-017-0518-2
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