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15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell
BACKGROUND: A great number of studies reported that 12/15-lipoxygenase (12/15-LO) played an important role in atherosclerosis. And its arachidonic acid(AA) metabolite, 15(S)-hydroperoxy-5,8,11,13-(Z,Z,Z,E)-eicosatetraenoic acid (15(S)-HETE), is demonstrated to mediate endothelial dysfunction. 15-oxo...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5508789/ https://www.ncbi.nlm.nih.gov/pubmed/28701173 http://dx.doi.org/10.1186/s12944-017-0518-2 |
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author | Ma, Guohua Pan, Bing Ren, Sufen Guo, Caixia Guo, Yansong Wei, Lixin Zheng, Lemin Chen, Buxing |
author_facet | Ma, Guohua Pan, Bing Ren, Sufen Guo, Caixia Guo, Yansong Wei, Lixin Zheng, Lemin Chen, Buxing |
author_sort | Ma, Guohua |
collection | PubMed |
description | BACKGROUND: A great number of studies reported that 12/15-lipoxygenase (12/15-LO) played an important role in atherosclerosis. And its arachidonic acid(AA) metabolite, 15(S)-hydroperoxy-5,8,11,13-(Z,Z,Z,E)-eicosatetraenoic acid (15(S)-HETE), is demonstrated to mediate endothelial dysfunction. 15-oxo-5,8,11,13-(Z,Z,Z,E)-eicosatetraenoic acid (15-oxo-ETE) was formed from 15-hydroxyprostaglandin dehydrogenase (PGDH)-mediated oxidation of 15(S)-HETE. However, relatively little is known about the biological effects of 15-oxo-ETE in cardiovascular disease. Here, we explore the likely role of 15-lipoxygenase (LO)-1-mediated AA metabolism,15-oxo-ETE, in the early pathogenesis of atherosclerosis. METHODS: The 15-oxo-ETE level in serum was detected by means of liquid chromatography and online tandem mass spectrometry (LC-MS/MS). And the underlying mechanisms were illuminated by molecular techniques, including immunoblotting, MTT assay, immunocytochemistry and Immunohistochemistry. RESULTS: Increased 15-oxo-ETE level is found in in patients with acute myocardial infarction (AMI). After 15-oxo-ETE treatment, Human umbilical vein endothelial cells (HUVECs) showed more attractive to monocytes, whereas monocyte adhesion is suppressed when treated with PKC inhibitor. In ex vivo study, exposure of arteries from C57 mice and ApoE−/−mice to 15-oxo-ETE led to significantly increased E-selectin expression and monocyte adhesion. CONCLUSIONS: This is the first report that 15-oxo-ETE promotes early pathological process of atherosclerosis by accelerating E-selectin expression and monocyte adhesion. 15-oxo-ETE -induced monocyte adhesion is partly attributable to activation of PKC. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12944-017-0518-2) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5508789 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-55087892017-07-17 15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell Ma, Guohua Pan, Bing Ren, Sufen Guo, Caixia Guo, Yansong Wei, Lixin Zheng, Lemin Chen, Buxing Lipids Health Dis Research BACKGROUND: A great number of studies reported that 12/15-lipoxygenase (12/15-LO) played an important role in atherosclerosis. And its arachidonic acid(AA) metabolite, 15(S)-hydroperoxy-5,8,11,13-(Z,Z,Z,E)-eicosatetraenoic acid (15(S)-HETE), is demonstrated to mediate endothelial dysfunction. 15-oxo-5,8,11,13-(Z,Z,Z,E)-eicosatetraenoic acid (15-oxo-ETE) was formed from 15-hydroxyprostaglandin dehydrogenase (PGDH)-mediated oxidation of 15(S)-HETE. However, relatively little is known about the biological effects of 15-oxo-ETE in cardiovascular disease. Here, we explore the likely role of 15-lipoxygenase (LO)-1-mediated AA metabolism,15-oxo-ETE, in the early pathogenesis of atherosclerosis. METHODS: The 15-oxo-ETE level in serum was detected by means of liquid chromatography and online tandem mass spectrometry (LC-MS/MS). And the underlying mechanisms were illuminated by molecular techniques, including immunoblotting, MTT assay, immunocytochemistry and Immunohistochemistry. RESULTS: Increased 15-oxo-ETE level is found in in patients with acute myocardial infarction (AMI). After 15-oxo-ETE treatment, Human umbilical vein endothelial cells (HUVECs) showed more attractive to monocytes, whereas monocyte adhesion is suppressed when treated with PKC inhibitor. In ex vivo study, exposure of arteries from C57 mice and ApoE−/−mice to 15-oxo-ETE led to significantly increased E-selectin expression and monocyte adhesion. CONCLUSIONS: This is the first report that 15-oxo-ETE promotes early pathological process of atherosclerosis by accelerating E-selectin expression and monocyte adhesion. 15-oxo-ETE -induced monocyte adhesion is partly attributable to activation of PKC. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12944-017-0518-2) contains supplementary material, which is available to authorized users. BioMed Central 2017-07-12 /pmc/articles/PMC5508789/ /pubmed/28701173 http://dx.doi.org/10.1186/s12944-017-0518-2 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Ma, Guohua Pan, Bing Ren, Sufen Guo, Caixia Guo, Yansong Wei, Lixin Zheng, Lemin Chen, Buxing 15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell |
title | 15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell |
title_full | 15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell |
title_fullStr | 15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell |
title_full_unstemmed | 15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell |
title_short | 15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell |
title_sort | 15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5508789/ https://www.ncbi.nlm.nih.gov/pubmed/28701173 http://dx.doi.org/10.1186/s12944-017-0518-2 |
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