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Patch clamp-assisted single neuron lipidomics

Our understanding of the physiological and pathological functions of brain lipids is limited by the inability to analyze these molecules at cellular resolution. Here, we present a method that enables the detection of lipids in identified single neurons from live mammalian brains. Neuronal cell bodie...

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Detalles Bibliográficos
Autores principales: Merrill, Collin B., Basit, Abdul, Armirotti, Andrea, Jia, Yousheng, Gall, Christine M., Lynch, Gary, Piomelli, Daniele
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5509708/
https://www.ncbi.nlm.nih.gov/pubmed/28706218
http://dx.doi.org/10.1038/s41598-017-05607-3
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author Merrill, Collin B.
Basit, Abdul
Armirotti, Andrea
Jia, Yousheng
Gall, Christine M.
Lynch, Gary
Piomelli, Daniele
author_facet Merrill, Collin B.
Basit, Abdul
Armirotti, Andrea
Jia, Yousheng
Gall, Christine M.
Lynch, Gary
Piomelli, Daniele
author_sort Merrill, Collin B.
collection PubMed
description Our understanding of the physiological and pathological functions of brain lipids is limited by the inability to analyze these molecules at cellular resolution. Here, we present a method that enables the detection of lipids in identified single neurons from live mammalian brains. Neuronal cell bodies are captured from perfused mouse brain slices by patch clamping, and lipids are analyzed using an optimized nanoflow liquid chromatography/mass spectrometry protocol. In a first application of the method, we identified more than 40 lipid species from dentate gyrus granule cells and CA1 pyramidal neurons of the hippocampus. This survey revealed substantial lipid profile differences between neurons and whole brain tissue, as well as between resting and physiologically stimulated neurons. The results suggest that patch clamp-assisted single neuron lipidomics could be broadly applied to investigate neuronal lipid homeostasis in healthy and diseased brains.
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spelling pubmed-55097082017-07-17 Patch clamp-assisted single neuron lipidomics Merrill, Collin B. Basit, Abdul Armirotti, Andrea Jia, Yousheng Gall, Christine M. Lynch, Gary Piomelli, Daniele Sci Rep Article Our understanding of the physiological and pathological functions of brain lipids is limited by the inability to analyze these molecules at cellular resolution. Here, we present a method that enables the detection of lipids in identified single neurons from live mammalian brains. Neuronal cell bodies are captured from perfused mouse brain slices by patch clamping, and lipids are analyzed using an optimized nanoflow liquid chromatography/mass spectrometry protocol. In a first application of the method, we identified more than 40 lipid species from dentate gyrus granule cells and CA1 pyramidal neurons of the hippocampus. This survey revealed substantial lipid profile differences between neurons and whole brain tissue, as well as between resting and physiologically stimulated neurons. The results suggest that patch clamp-assisted single neuron lipidomics could be broadly applied to investigate neuronal lipid homeostasis in healthy and diseased brains. Nature Publishing Group UK 2017-07-13 /pmc/articles/PMC5509708/ /pubmed/28706218 http://dx.doi.org/10.1038/s41598-017-05607-3 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Merrill, Collin B.
Basit, Abdul
Armirotti, Andrea
Jia, Yousheng
Gall, Christine M.
Lynch, Gary
Piomelli, Daniele
Patch clamp-assisted single neuron lipidomics
title Patch clamp-assisted single neuron lipidomics
title_full Patch clamp-assisted single neuron lipidomics
title_fullStr Patch clamp-assisted single neuron lipidomics
title_full_unstemmed Patch clamp-assisted single neuron lipidomics
title_short Patch clamp-assisted single neuron lipidomics
title_sort patch clamp-assisted single neuron lipidomics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5509708/
https://www.ncbi.nlm.nih.gov/pubmed/28706218
http://dx.doi.org/10.1038/s41598-017-05607-3
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