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Brain specific Lamellipodin knockout results in hyperactivity and increased anxiety of mice

Lamellipodin (Lpd) functions as an important signalling integrator downstream of growth factor and axon guidance receptors. Mechanistically, Lpd promotes actin polymerization by interacting with F-actin and the actin effectors Ena/VASP proteins and the SCAR/WAVE complex. Thereby, Lpd supports lamell...

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Autores principales: Bodo, Cristian, Fernandes, Cathy, Krause, Matthias
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5511208/
https://www.ncbi.nlm.nih.gov/pubmed/28710397
http://dx.doi.org/10.1038/s41598-017-05043-3
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author Bodo, Cristian
Fernandes, Cathy
Krause, Matthias
author_facet Bodo, Cristian
Fernandes, Cathy
Krause, Matthias
author_sort Bodo, Cristian
collection PubMed
description Lamellipodin (Lpd) functions as an important signalling integrator downstream of growth factor and axon guidance receptors. Mechanistically, Lpd promotes actin polymerization by interacting with F-actin and the actin effectors Ena/VASP proteins and the SCAR/WAVE complex. Thereby, Lpd supports lamellipodia protrusion, cell migration and endocytosis. In the mammalian central nervous system, Lpd contributes to neuronal morphogenesis, neuronal migration during development and its C. elegans orthologue MIG-10 also supports synaptogenesis. However, the consequences of loss of Lpd in the CNS on behaviour are unknown. In our current study, we crossed our Lpd conditional knockout mice with a mouse line expressing Cre under the CNS specific Nestin promoter to restrict the genetic ablation of Lpd to the central nervous system. Detailed behavioural analysis of the resulting Nestin-Cre-Lpd knockout mouse line revealed a specific behavioural phenotype characterised by hyperactivity and increased anxiety.
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spelling pubmed-55112082017-07-17 Brain specific Lamellipodin knockout results in hyperactivity and increased anxiety of mice Bodo, Cristian Fernandes, Cathy Krause, Matthias Sci Rep Article Lamellipodin (Lpd) functions as an important signalling integrator downstream of growth factor and axon guidance receptors. Mechanistically, Lpd promotes actin polymerization by interacting with F-actin and the actin effectors Ena/VASP proteins and the SCAR/WAVE complex. Thereby, Lpd supports lamellipodia protrusion, cell migration and endocytosis. In the mammalian central nervous system, Lpd contributes to neuronal morphogenesis, neuronal migration during development and its C. elegans orthologue MIG-10 also supports synaptogenesis. However, the consequences of loss of Lpd in the CNS on behaviour are unknown. In our current study, we crossed our Lpd conditional knockout mice with a mouse line expressing Cre under the CNS specific Nestin promoter to restrict the genetic ablation of Lpd to the central nervous system. Detailed behavioural analysis of the resulting Nestin-Cre-Lpd knockout mouse line revealed a specific behavioural phenotype characterised by hyperactivity and increased anxiety. Nature Publishing Group UK 2017-07-14 /pmc/articles/PMC5511208/ /pubmed/28710397 http://dx.doi.org/10.1038/s41598-017-05043-3 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Bodo, Cristian
Fernandes, Cathy
Krause, Matthias
Brain specific Lamellipodin knockout results in hyperactivity and increased anxiety of mice
title Brain specific Lamellipodin knockout results in hyperactivity and increased anxiety of mice
title_full Brain specific Lamellipodin knockout results in hyperactivity and increased anxiety of mice
title_fullStr Brain specific Lamellipodin knockout results in hyperactivity and increased anxiety of mice
title_full_unstemmed Brain specific Lamellipodin knockout results in hyperactivity and increased anxiety of mice
title_short Brain specific Lamellipodin knockout results in hyperactivity and increased anxiety of mice
title_sort brain specific lamellipodin knockout results in hyperactivity and increased anxiety of mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5511208/
https://www.ncbi.nlm.nih.gov/pubmed/28710397
http://dx.doi.org/10.1038/s41598-017-05043-3
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