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An att site-based recombination reporter system for genome engineering and synthetic DNA assembly
BACKGROUND: Direct manipulation of the genome is a widespread technique for genetic studies and synthetic biology applications. The tyrosine and serine site-specific recombination systems of bacteriophages HK022 and ΦC31 are widely used for stable directional exchange and relocation of DNA sequences...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5512741/ https://www.ncbi.nlm.nih.gov/pubmed/28705159 http://dx.doi.org/10.1186/s12896-017-0382-1 |