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Avoidance of reporter assay distortions from fused dual reporters

Positioning test sequences between fused reporters permits monitoring of both translation levels and framing, before and after the test sequence. Many studies, including those on recoding such as productive ribosomal frameshifting and stop codon readthrough, use distinguishable luciferases or fluore...

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Detalles Bibliográficos
Autores principales: Loughran, Gary, Howard, Michael T., Firth, Andrew E., Atkins, John F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5513072/
https://www.ncbi.nlm.nih.gov/pubmed/28442579
http://dx.doi.org/10.1261/rna.061051.117