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Assessment of isomiR Discrimination Using Commercial qPCR Methods

We sought to determine whether commercial quantitative polymerase chain reaction (qPCR) methods are capable of distinguishing isomiRs: variants of mature microRNAs (miRNAs) with sequence endpoint differences. We used two commercially available miRNA qPCR methods to quantify miR-21-5p in both synthet...

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Detalles Bibliográficos
Autores principales: Magee, Rogan, Telonis, Aristeidis G., Cherlin, Tess, Rigoutsos, Isidore, Londin, Eric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5514785/
https://www.ncbi.nlm.nih.gov/pubmed/28730153
http://dx.doi.org/10.3390/ncrna3020018