Oligo- and dsDNA-mediated genome editing using a tetA dual selection system in Escherichia coli

The ability to precisely and seamlessly modify a target genome is needed for metabolic engineering and synthetic biology techniques aimed at creating potent biosystems. Herein, we report on a promising method in Escherichia coli that relies on the insertion of an optimized tetA dual selection casset...

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Detalles Bibliográficos
Autores principales: Ryu, Young Shin, Chandran, Sathesh-Prabu, Kim, Kyungchul, Lee, Sung Kuk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5515457/
https://www.ncbi.nlm.nih.gov/pubmed/28719630
http://dx.doi.org/10.1371/journal.pone.0181501

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5515457/
https://www.ncbi.nlm.nih.gov/pubmed/28719630
http://dx.doi.org/10.1371/journal.pone.0181501

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