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Oligo- and dsDNA-mediated genome editing using a tetA dual selection system in Escherichia coli
The ability to precisely and seamlessly modify a target genome is needed for metabolic engineering and synthetic biology techniques aimed at creating potent biosystems. Herein, we report on a promising method in Escherichia coli that relies on the insertion of an optimized tetA dual selection casset...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5515457/ https://www.ncbi.nlm.nih.gov/pubmed/28719630 http://dx.doi.org/10.1371/journal.pone.0181501 |