Comparison of DNA-, PMA-, and RNA-based 16S rRNA Illumina sequencing for detection of live bacteria in water

The limitation of 16S rRNA gene sequencing (DNA-based) for microbial community analyses in water is the inability to differentiate live (dormant cells as well as growing or non-growing metabolically active cells) and dead cells, which can lead to false positive results in the absence of live microbe...

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Detalles Bibliográficos
Autores principales: Li, Ru, Tun, Hein Min, Jahan, Musarrat, Zhang, Zhengxiao, Kumar, Ayush, Dilantha Fernando, W. G., Farenhorst, Annemieke, Khafipour, Ehsan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5515937/
https://www.ncbi.nlm.nih.gov/pubmed/28720878
http://dx.doi.org/10.1038/s41598-017-02516-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5515937/
https://www.ncbi.nlm.nih.gov/pubmed/28720878
http://dx.doi.org/10.1038/s41598-017-02516-3

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