Expression pattern of CD11c on lung immune cells after disseminated murine cytomegalovirus infection

BACKGROUND: Cytomegalovirus (CMV) infection occurs frequently and is widespread globally. Numerous studies have shown that various types of immune cells play roles in mediating the response to CMV infection. CD11c, a commonly used dendritic cell (DC) marker, is expressed by other immune cells as wel...

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Autores principales: Liao, Yi, Liu, Xinglou, Huang, Yuan, Huang, Heyu, Lu, Yuanyuan, Zhang, Yanan, Shu, Sainan, Fang, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5516330/
https://www.ncbi.nlm.nih.gov/pubmed/28720115
http://dx.doi.org/10.1186/s12985-017-0801-x
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author Liao, Yi
Liu, Xinglou
Huang, Yuan
Huang, Heyu
Lu, Yuanyuan
Zhang, Yanan
Shu, Sainan
Fang, Feng
author_facet Liao, Yi
Liu, Xinglou
Huang, Yuan
Huang, Heyu
Lu, Yuanyuan
Zhang, Yanan
Shu, Sainan
Fang, Feng
author_sort Liao, Yi
collection PubMed
description BACKGROUND: Cytomegalovirus (CMV) infection occurs frequently and is widespread globally. Numerous studies have shown that various types of immune cells play roles in mediating the response to CMV infection. CD11c, a commonly used dendritic cell (DC) marker, is expressed by other immune cells as well, such as T cells. This study analyzed the immune cells that express CD11c and monitored the expression level of their specific cell surface markers in the lung following a disseminated murine (M)CMV infection. METHODS: Mouse models of disseminated MCMV infection were used; uninfected and lipopolysaccharide (LPS)-treated mice were used as controls. At 1, 3 and 7 days following infection, single cell suspensions prepared from freshly digested lung tissue were stained for CD11c, CD86 and MHC II. Stained cells were analyzed using flow cytometry. Peripheral blood and single cell suspensions from spleen were sorted as well. Then these cells were subjected to analyze the CD11c expression pattern on natural killer (NK) cells and T cells. RESULTS: This assay showed that after MCMV infection, the expression of CD86 on pulmonary CD11c(hi)MHC-II(hi) cells (encompassing conventional DCs) was higher at 3 days post-infection than at 1 or 7 days post-infection, accompanied by a downregulation of MHC II. In addition, expression of CD11c was greatly increased in the MCMV infection group at 7 days post infection. This study also detected a large population of cells displaying an intermediate level of expression of CD11c (CD11c(int)); these cells were in the MCMV groups exclusively, and were subsequently identified as CD8(+) T cells. In lung, spleen and blood, different proportions of CD11c(int) cells among the NK cell and T cell populations were observed between the BALB/c and C57BL/6 mice with or without MCMV infection. The expression level of NKp46 in NK cells dropped to a lower level after MCMV infection. CONCLUSIONS: The findings collectively indicate that CD11c(int)CD8(+) T cells might play a key role in anti-MCMV adaptive immune response in lungs, as well as in spleen and blood. B220(+)CD11c(int) NK cells might be a more effective type of NK cell, participating in anti-MCMV infection. The downregulation of NKp46, in particular, might be linked with the immune evasion of MCMV. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12985-017-0801-x) contains supplementary material, which is available to authorized users.
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spelling pubmed-55163302017-07-20 Expression pattern of CD11c on lung immune cells after disseminated murine cytomegalovirus infection Liao, Yi Liu, Xinglou Huang, Yuan Huang, Heyu Lu, Yuanyuan Zhang, Yanan Shu, Sainan Fang, Feng Virol J Research BACKGROUND: Cytomegalovirus (CMV) infection occurs frequently and is widespread globally. Numerous studies have shown that various types of immune cells play roles in mediating the response to CMV infection. CD11c, a commonly used dendritic cell (DC) marker, is expressed by other immune cells as well, such as T cells. This study analyzed the immune cells that express CD11c and monitored the expression level of their specific cell surface markers in the lung following a disseminated murine (M)CMV infection. METHODS: Mouse models of disseminated MCMV infection were used; uninfected and lipopolysaccharide (LPS)-treated mice were used as controls. At 1, 3 and 7 days following infection, single cell suspensions prepared from freshly digested lung tissue were stained for CD11c, CD86 and MHC II. Stained cells were analyzed using flow cytometry. Peripheral blood and single cell suspensions from spleen were sorted as well. Then these cells were subjected to analyze the CD11c expression pattern on natural killer (NK) cells and T cells. RESULTS: This assay showed that after MCMV infection, the expression of CD86 on pulmonary CD11c(hi)MHC-II(hi) cells (encompassing conventional DCs) was higher at 3 days post-infection than at 1 or 7 days post-infection, accompanied by a downregulation of MHC II. In addition, expression of CD11c was greatly increased in the MCMV infection group at 7 days post infection. This study also detected a large population of cells displaying an intermediate level of expression of CD11c (CD11c(int)); these cells were in the MCMV groups exclusively, and were subsequently identified as CD8(+) T cells. In lung, spleen and blood, different proportions of CD11c(int) cells among the NK cell and T cell populations were observed between the BALB/c and C57BL/6 mice with or without MCMV infection. The expression level of NKp46 in NK cells dropped to a lower level after MCMV infection. CONCLUSIONS: The findings collectively indicate that CD11c(int)CD8(+) T cells might play a key role in anti-MCMV adaptive immune response in lungs, as well as in spleen and blood. B220(+)CD11c(int) NK cells might be a more effective type of NK cell, participating in anti-MCMV infection. The downregulation of NKp46, in particular, might be linked with the immune evasion of MCMV. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12985-017-0801-x) contains supplementary material, which is available to authorized users. BioMed Central 2017-07-18 /pmc/articles/PMC5516330/ /pubmed/28720115 http://dx.doi.org/10.1186/s12985-017-0801-x Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Liao, Yi
Liu, Xinglou
Huang, Yuan
Huang, Heyu
Lu, Yuanyuan
Zhang, Yanan
Shu, Sainan
Fang, Feng
Expression pattern of CD11c on lung immune cells after disseminated murine cytomegalovirus infection
title Expression pattern of CD11c on lung immune cells after disseminated murine cytomegalovirus infection
title_full Expression pattern of CD11c on lung immune cells after disseminated murine cytomegalovirus infection
title_fullStr Expression pattern of CD11c on lung immune cells after disseminated murine cytomegalovirus infection
title_full_unstemmed Expression pattern of CD11c on lung immune cells after disseminated murine cytomegalovirus infection
title_short Expression pattern of CD11c on lung immune cells after disseminated murine cytomegalovirus infection
title_sort expression pattern of cd11c on lung immune cells after disseminated murine cytomegalovirus infection
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5516330/
https://www.ncbi.nlm.nih.gov/pubmed/28720115
http://dx.doi.org/10.1186/s12985-017-0801-x
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