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Cell Counting and Viability Assessment of 2D and 3D Cell Cultures: Expected Reliability of the Trypan Blue Assay

BACKGROUND: Whatever the target of an experiment in cell biology, cell counting and viability assessment are always computed. The Trypan Blue (TB) assay was proposed about a century ago and is still the most widely used method to perform cell viability analysis. Furthermore, the combined use of TB w...

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Detalles Bibliográficos
Autores principales: Piccinini, Filippo, Tesei, Anna, Arienti, Chiara, Bevilacqua, Alessandro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5518102/
https://www.ncbi.nlm.nih.gov/pubmed/28814944
http://dx.doi.org/10.1186/s12575-017-0056-3