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An in vitro system for the leader-primed transcription of coronavirus mRNAs.

We have developed an in vitro transcription system which can utilize exogenous leader RNA for mouse hepatitis virus (MHV) 'leader-primed' mRNA transcription. Cytoplasmic extracts containing viral proteins and template RNA were prepared by lysolecithin permeabilization of MHV-infected cells...

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Detalles Bibliográficos
Autores principales: Baker, S C, Lai, M M
Formato: Texto
Lenguaje:English
Publicado: 1990
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC552193/
https://www.ncbi.nlm.nih.gov/pubmed/2174358