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Frame shift mutations as a novel mechanism for the generation of neutralization resistant mutants of human respiratory syncytial virus.

The genetic characterization of four previously reported mutants of human respiratory syncytial (RS) virus resistant to monoclonal antibody 63G is described. Sequences of the G protein genes were obtained from: (i) mRNA derived cDNA recombinants, (ii) direct mRNA sequencing and (iii) amplified vRNA...

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Detalles Bibliográficos
Autores principales: García-Barreno, B, Portela, A, Delgado, T, López, J A, Melero, J A
Formato: Texto
Lenguaje:English
Publicado: 1990
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC552194/
https://www.ncbi.nlm.nih.gov/pubmed/2249671
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author García-Barreno, B
Portela, A
Delgado, T
López, J A
Melero, J A
author_facet García-Barreno, B
Portela, A
Delgado, T
López, J A
Melero, J A
author_sort García-Barreno, B
collection PubMed
description The genetic characterization of four previously reported mutants of human respiratory syncytial (RS) virus resistant to monoclonal antibody 63G is described. Sequences of the G protein genes were obtained from: (i) mRNA derived cDNA recombinants, (ii) direct mRNA sequencing and (iii) amplified vRNA derived cDNAs. The results obtained indicate that the original escape mutants, recovered from individual plaques, contained heterogeneous viral populations. This heterogeneity affected the number of adenosine residues present after nucleotides 588 or 623 of the G protein gene. Mutant viruses recovered after a second plaque purification step generated homogeneous sequences but contained single adenosine insertions or deletions at those two sites compared with the Long sequence. These genetic alterations introduced frameshift changes which are reflected in both the antigenic and structural properties of the mutant G proteins. The origin and importance of frameshift mutations in the RS virus G protein gene are discussed.
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spelling pubmed-5521942005-04-27 Frame shift mutations as a novel mechanism for the generation of neutralization resistant mutants of human respiratory syncytial virus. García-Barreno, B Portela, A Delgado, T López, J A Melero, J A EMBO J Research Article The genetic characterization of four previously reported mutants of human respiratory syncytial (RS) virus resistant to monoclonal antibody 63G is described. Sequences of the G protein genes were obtained from: (i) mRNA derived cDNA recombinants, (ii) direct mRNA sequencing and (iii) amplified vRNA derived cDNAs. The results obtained indicate that the original escape mutants, recovered from individual plaques, contained heterogeneous viral populations. This heterogeneity affected the number of adenosine residues present after nucleotides 588 or 623 of the G protein gene. Mutant viruses recovered after a second plaque purification step generated homogeneous sequences but contained single adenosine insertions or deletions at those two sites compared with the Long sequence. These genetic alterations introduced frameshift changes which are reflected in both the antigenic and structural properties of the mutant G proteins. The origin and importance of frameshift mutations in the RS virus G protein gene are discussed. 1990-12 /pmc/articles/PMC552194/ /pubmed/2249671 Text en
spellingShingle Research Article
García-Barreno, B
Portela, A
Delgado, T
López, J A
Melero, J A
Frame shift mutations as a novel mechanism for the generation of neutralization resistant mutants of human respiratory syncytial virus.
title Frame shift mutations as a novel mechanism for the generation of neutralization resistant mutants of human respiratory syncytial virus.
title_full Frame shift mutations as a novel mechanism for the generation of neutralization resistant mutants of human respiratory syncytial virus.
title_fullStr Frame shift mutations as a novel mechanism for the generation of neutralization resistant mutants of human respiratory syncytial virus.
title_full_unstemmed Frame shift mutations as a novel mechanism for the generation of neutralization resistant mutants of human respiratory syncytial virus.
title_short Frame shift mutations as a novel mechanism for the generation of neutralization resistant mutants of human respiratory syncytial virus.
title_sort frame shift mutations as a novel mechanism for the generation of neutralization resistant mutants of human respiratory syncytial virus.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC552194/
https://www.ncbi.nlm.nih.gov/pubmed/2249671
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