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Charge reversible calcium phosphate lipid hybrid nanoparticle for siRNA delivery
Bcl-2 gene is an important target to treat lung cancer. The small interference RNA (siRNA) of Bcl-2 gene (siBcl-2) can specifically silence Bcl-2 gene. However, naked siBcl-2 is difficult to accumulate in the tumor tissue to exert its activity. In this paper, a calcium phosphate lipid hybrid nanopar...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5522105/ https://www.ncbi.nlm.nih.gov/pubmed/28514759 http://dx.doi.org/10.18632/oncotarget.17484 |
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author | Cai, Rong-Qiao Liu, Dao-Zhou Cui, Han Cheng, Ying Liu, Miao Zhang, Bang-Le Mei, Qi-Bing Zhou, Si-Yuan |
author_facet | Cai, Rong-Qiao Liu, Dao-Zhou Cui, Han Cheng, Ying Liu, Miao Zhang, Bang-Le Mei, Qi-Bing Zhou, Si-Yuan |
author_sort | Cai, Rong-Qiao |
collection | PubMed |
description | Bcl-2 gene is an important target to treat lung cancer. The small interference RNA (siRNA) of Bcl-2 gene (siBcl-2) can specifically silence Bcl-2 gene. However, naked siBcl-2 is difficult to accumulate in the tumor tissue to exert its activity. In this paper, a calcium phosphate lipid hybrid nanoparticle that possessed charge reversible property was prepared to enhance the activity of siBcl-2 in vivo. The average diameter and zeta potential of siBcl-2 loaded calcium phosphate lipid hybrid nanoparticles (LNPS@siBcl-2) were 80 nm and −13 mV at pH7.4 whereas the diameter and zeta potential changed to 1506 nm and +9 mV at pH5.0. LNPS@siBcl-2 could efficiently deliver siBcl-2 to the cytoplasm and significantly decreased the expression of Bcl-2 in A549 cells. Moreover, the in vivo experimental results showed that most of the Cy5-siBcl-2 accumulated in tumor tissue after LNPS@Cy5-siBcl-2 was administered to tumor-bearing mice by tail vein injection. Meanwhile, the expression of Bcl-2 was decreased but the expression of the BAX and Caspase-3 was increased in tumor tissue. LNPS@siBcl-2 significantly inhibited the growth of tumor in tumor-bearing mice without any obvious systemic toxicity. Thus, the charge reversible calcium phosphate lipid hybrid nanoparticle was an excellent siBcl-2 delivery carrier to improve the activity of siBcl-2 in vivo. LNPS@siBcl-2 has potential in the treatment of lung cancer. |
format | Online Article Text |
id | pubmed-5522105 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-55221052017-08-08 Charge reversible calcium phosphate lipid hybrid nanoparticle for siRNA delivery Cai, Rong-Qiao Liu, Dao-Zhou Cui, Han Cheng, Ying Liu, Miao Zhang, Bang-Le Mei, Qi-Bing Zhou, Si-Yuan Oncotarget Research Paper Bcl-2 gene is an important target to treat lung cancer. The small interference RNA (siRNA) of Bcl-2 gene (siBcl-2) can specifically silence Bcl-2 gene. However, naked siBcl-2 is difficult to accumulate in the tumor tissue to exert its activity. In this paper, a calcium phosphate lipid hybrid nanoparticle that possessed charge reversible property was prepared to enhance the activity of siBcl-2 in vivo. The average diameter and zeta potential of siBcl-2 loaded calcium phosphate lipid hybrid nanoparticles (LNPS@siBcl-2) were 80 nm and −13 mV at pH7.4 whereas the diameter and zeta potential changed to 1506 nm and +9 mV at pH5.0. LNPS@siBcl-2 could efficiently deliver siBcl-2 to the cytoplasm and significantly decreased the expression of Bcl-2 in A549 cells. Moreover, the in vivo experimental results showed that most of the Cy5-siBcl-2 accumulated in tumor tissue after LNPS@Cy5-siBcl-2 was administered to tumor-bearing mice by tail vein injection. Meanwhile, the expression of Bcl-2 was decreased but the expression of the BAX and Caspase-3 was increased in tumor tissue. LNPS@siBcl-2 significantly inhibited the growth of tumor in tumor-bearing mice without any obvious systemic toxicity. Thus, the charge reversible calcium phosphate lipid hybrid nanoparticle was an excellent siBcl-2 delivery carrier to improve the activity of siBcl-2 in vivo. LNPS@siBcl-2 has potential in the treatment of lung cancer. Impact Journals LLC 2017-04-27 /pmc/articles/PMC5522105/ /pubmed/28514759 http://dx.doi.org/10.18632/oncotarget.17484 Text en Copyright: © 2017 Cai et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Research Paper Cai, Rong-Qiao Liu, Dao-Zhou Cui, Han Cheng, Ying Liu, Miao Zhang, Bang-Le Mei, Qi-Bing Zhou, Si-Yuan Charge reversible calcium phosphate lipid hybrid nanoparticle for siRNA delivery |
title | Charge reversible calcium phosphate lipid hybrid nanoparticle for siRNA delivery |
title_full | Charge reversible calcium phosphate lipid hybrid nanoparticle for siRNA delivery |
title_fullStr | Charge reversible calcium phosphate lipid hybrid nanoparticle for siRNA delivery |
title_full_unstemmed | Charge reversible calcium phosphate lipid hybrid nanoparticle for siRNA delivery |
title_short | Charge reversible calcium phosphate lipid hybrid nanoparticle for siRNA delivery |
title_sort | charge reversible calcium phosphate lipid hybrid nanoparticle for sirna delivery |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5522105/ https://www.ncbi.nlm.nih.gov/pubmed/28514759 http://dx.doi.org/10.18632/oncotarget.17484 |
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