Cargando…

Caveolin-1 scaffolding domain peptides enhance anti-inflammatory effect of heme oxygenase-1 through interrupting its interact with caveolin-1

Caveolin-1(Cav-1) scaffolding domain (CSD) peptides compete with the plasma membrane Cav-1, inhibit the interaction of the proteins and Cav-1, and re-store the functions of Cav-1 binding proteins. Heme oxygenase-1 (HO-1) binds to Cav-1 and its enzymatic activity was inhibited. In this study, we inve...

Descripción completa

Detalles Bibliográficos
Autores principales: Weng, Ping, Zhang, Xiao-Tong, Sheng, Qiong, Tian, Wen-Fang, Chen, Jun-Liang, Yuan, Jia-Jia, Zhang, Ji-Ru, Pang, Qing-Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5522314/
https://www.ncbi.nlm.nih.gov/pubmed/28402952
http://dx.doi.org/10.18632/oncotarget.16676
_version_ 1783252147636273152
author Weng, Ping
Zhang, Xiao-Tong
Sheng, Qiong
Tian, Wen-Fang
Chen, Jun-Liang
Yuan, Jia-Jia
Zhang, Ji-Ru
Pang, Qing-Feng
author_facet Weng, Ping
Zhang, Xiao-Tong
Sheng, Qiong
Tian, Wen-Fang
Chen, Jun-Liang
Yuan, Jia-Jia
Zhang, Ji-Ru
Pang, Qing-Feng
author_sort Weng, Ping
collection PubMed
description Caveolin-1(Cav-1) scaffolding domain (CSD) peptides compete with the plasma membrane Cav-1, inhibit the interaction of the proteins and Cav-1, and re-store the functions of Cav-1 binding proteins. Heme oxygenase-1 (HO-1) binds to Cav-1 and its enzymatic activity was inhibited. In this study, we investigated the effect of CSD peptides on interaction between HO-1 and Cav-1, and on the HO-1 activity in vitro and in vivo. Our data showed that CSD peptides decreased the compartmentalization of HO-1 and Cav-1, and increased the HO-1 activity both in LPS-treated alveolar macrophages and in mice. Meanwhile, CSD peptides obviously ameliorated the pathology changes in mice and lowered the following injury indexes: the wet/dry ratio of lung tissues, total cell numbers in bronchoalveolar lavage fluid and lactate dehydrogenase activity in the serum. Mechanistically, it was firstly found that CSD peptides promoted alveolar macrophages polarization to M2 phenotype and inhibited the IκB degeneration. Furthermore, CSD peptides down-regulated the expression of IL-1β, IL-6, TNF-α, MCP-1, and iNOS in alveolar macrophages and in lung tissue. However, the protective role of CSD peptides on LPS-induced acute lung injury in mice could be abolished by zinc protoporphyrin IX (ZnPP, a HO-1 activity inhibitor). In summary, CSD peptides have beneficial anti-inflammatory effects by restoring the HO-1 activity suppressed by Cav-1 on plasma membrane.
format Online
Article
Text
id pubmed-5522314
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Impact Journals LLC
record_format MEDLINE/PubMed
spelling pubmed-55223142017-08-21 Caveolin-1 scaffolding domain peptides enhance anti-inflammatory effect of heme oxygenase-1 through interrupting its interact with caveolin-1 Weng, Ping Zhang, Xiao-Tong Sheng, Qiong Tian, Wen-Fang Chen, Jun-Liang Yuan, Jia-Jia Zhang, Ji-Ru Pang, Qing-Feng Oncotarget Research Paper Caveolin-1(Cav-1) scaffolding domain (CSD) peptides compete with the plasma membrane Cav-1, inhibit the interaction of the proteins and Cav-1, and re-store the functions of Cav-1 binding proteins. Heme oxygenase-1 (HO-1) binds to Cav-1 and its enzymatic activity was inhibited. In this study, we investigated the effect of CSD peptides on interaction between HO-1 and Cav-1, and on the HO-1 activity in vitro and in vivo. Our data showed that CSD peptides decreased the compartmentalization of HO-1 and Cav-1, and increased the HO-1 activity both in LPS-treated alveolar macrophages and in mice. Meanwhile, CSD peptides obviously ameliorated the pathology changes in mice and lowered the following injury indexes: the wet/dry ratio of lung tissues, total cell numbers in bronchoalveolar lavage fluid and lactate dehydrogenase activity in the serum. Mechanistically, it was firstly found that CSD peptides promoted alveolar macrophages polarization to M2 phenotype and inhibited the IκB degeneration. Furthermore, CSD peptides down-regulated the expression of IL-1β, IL-6, TNF-α, MCP-1, and iNOS in alveolar macrophages and in lung tissue. However, the protective role of CSD peptides on LPS-induced acute lung injury in mice could be abolished by zinc protoporphyrin IX (ZnPP, a HO-1 activity inhibitor). In summary, CSD peptides have beneficial anti-inflammatory effects by restoring the HO-1 activity suppressed by Cav-1 on plasma membrane. Impact Journals LLC 2017-03-29 /pmc/articles/PMC5522314/ /pubmed/28402952 http://dx.doi.org/10.18632/oncotarget.16676 Text en Copyright: © 2017 Weng et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (http://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Weng, Ping
Zhang, Xiao-Tong
Sheng, Qiong
Tian, Wen-Fang
Chen, Jun-Liang
Yuan, Jia-Jia
Zhang, Ji-Ru
Pang, Qing-Feng
Caveolin-1 scaffolding domain peptides enhance anti-inflammatory effect of heme oxygenase-1 through interrupting its interact with caveolin-1
title Caveolin-1 scaffolding domain peptides enhance anti-inflammatory effect of heme oxygenase-1 through interrupting its interact with caveolin-1
title_full Caveolin-1 scaffolding domain peptides enhance anti-inflammatory effect of heme oxygenase-1 through interrupting its interact with caveolin-1
title_fullStr Caveolin-1 scaffolding domain peptides enhance anti-inflammatory effect of heme oxygenase-1 through interrupting its interact with caveolin-1
title_full_unstemmed Caveolin-1 scaffolding domain peptides enhance anti-inflammatory effect of heme oxygenase-1 through interrupting its interact with caveolin-1
title_short Caveolin-1 scaffolding domain peptides enhance anti-inflammatory effect of heme oxygenase-1 through interrupting its interact with caveolin-1
title_sort caveolin-1 scaffolding domain peptides enhance anti-inflammatory effect of heme oxygenase-1 through interrupting its interact with caveolin-1
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5522314/
https://www.ncbi.nlm.nih.gov/pubmed/28402952
http://dx.doi.org/10.18632/oncotarget.16676
work_keys_str_mv AT wengping caveolin1scaffoldingdomainpeptidesenhanceantiinflammatoryeffectofhemeoxygenase1throughinterruptingitsinteractwithcaveolin1
AT zhangxiaotong caveolin1scaffoldingdomainpeptidesenhanceantiinflammatoryeffectofhemeoxygenase1throughinterruptingitsinteractwithcaveolin1
AT shengqiong caveolin1scaffoldingdomainpeptidesenhanceantiinflammatoryeffectofhemeoxygenase1throughinterruptingitsinteractwithcaveolin1
AT tianwenfang caveolin1scaffoldingdomainpeptidesenhanceantiinflammatoryeffectofhemeoxygenase1throughinterruptingitsinteractwithcaveolin1
AT chenjunliang caveolin1scaffoldingdomainpeptidesenhanceantiinflammatoryeffectofhemeoxygenase1throughinterruptingitsinteractwithcaveolin1
AT yuanjiajia caveolin1scaffoldingdomainpeptidesenhanceantiinflammatoryeffectofhemeoxygenase1throughinterruptingitsinteractwithcaveolin1
AT zhangjiru caveolin1scaffoldingdomainpeptidesenhanceantiinflammatoryeffectofhemeoxygenase1throughinterruptingitsinteractwithcaveolin1
AT pangqingfeng caveolin1scaffoldingdomainpeptidesenhanceantiinflammatoryeffectofhemeoxygenase1throughinterruptingitsinteractwithcaveolin1