Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system

Precise genetic modifications in model animals are essential for biomedical research. Here, we report a programmable “base editing” system to induce precise base conversion with high efficiency in zebrafish. Using cytidine deaminase fused to Cas9 nickase, up to 28% of site-specific single-base mutat...

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Detalles Bibliográficos
Autores principales: Zhang, Yihan, Qin, Wei, Lu, Xiaochan, Xu, Jason, Huang, Haigen, Bai, Haipeng, Li, Song, Lin, Shuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5524635/
https://www.ncbi.nlm.nih.gov/pubmed/28740134
http://dx.doi.org/10.1038/s41467-017-00175-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5524635/
https://www.ncbi.nlm.nih.gov/pubmed/28740134
http://dx.doi.org/10.1038/s41467-017-00175-6

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