Freeze-drying of mammalian cells using trehalose: preservation of DNA integrity

The aim of this study was to investigate preservation of biomolecular structures, particularly DNA, in freeze-dried fibroblasts, after loading with trehalose via freezing-induced uptake. Cells were freeze-dried with trehalose alone or in a mixture of albumin and trehalose. Albumin was added to incre...

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Detalles Bibliográficos
Autores principales: Zhang, Miao, Oldenhof, Harriëtte, Sydykov, Bulat, Bigalk, Judith, Sieme, Harald, Wolkers, Willem F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5524761/
https://www.ncbi.nlm.nih.gov/pubmed/28740099
http://dx.doi.org/10.1038/s41598-017-06542-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5524761/
https://www.ncbi.nlm.nih.gov/pubmed/28740099
http://dx.doi.org/10.1038/s41598-017-06542-z

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