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Freeze-drying of mammalian cells using trehalose: preservation of DNA integrity
The aim of this study was to investigate preservation of biomolecular structures, particularly DNA, in freeze-dried fibroblasts, after loading with trehalose via freezing-induced uptake. Cells were freeze-dried with trehalose alone or in a mixture of albumin and trehalose. Albumin was added to incre...
Autores principales: | Zhang, Miao, Oldenhof, Harriëtte, Sydykov, Bulat, Bigalk, Judith, Sieme, Harald, Wolkers, Willem F. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5524761/ https://www.ncbi.nlm.nih.gov/pubmed/28740099 http://dx.doi.org/10.1038/s41598-017-06542-z |
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