Production of a recombinant phospholipase A2 in Escherichia coli using resonant acoustic mixing that improves oxygen transfer in shake flasks

BACKGROUND: Shake flasks are widely used during the development of bioprocesses for recombinant proteins. Cultures of recombinant Escherichia coli with orbital mixing (OM) have an oxygen limitation negatively affecting biomass growth and recombinant-protein production. With the aim to improve mixing...

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Detalles Bibliográficos
Autores principales: Valdez-Cruz, Norma A., Reynoso-Cereceda, Greta I., Pérez-Rodriguez, Saumel, Restrepo-Pineda, Sara, González-Santana, Jesus, Olvera, Alejandro, Zavala, Guadalupe, Alagón, Alejandro, Trujillo-Roldán, Mauricio A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5526256/
https://www.ncbi.nlm.nih.gov/pubmed/28743267
http://dx.doi.org/10.1186/s12934-017-0746-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5526256/
https://www.ncbi.nlm.nih.gov/pubmed/28743267
http://dx.doi.org/10.1186/s12934-017-0746-1

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