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Functional Characterization of SlSAHH2 in Tomato Fruit Ripening

S-adenosylhomocysteine hydrolase (SAHH) functions as an enzyme catalyzing the reversible hydrolysis of S-adenosylhomocysteine to homocysteine and adenosine. In the present work we have investigated its role in the ripening process of tomato fruit. Among the three SlSAHH genes we demonstrated that Sl...

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Autores principales: Yang, Lu, Hu, Guojian, Li, Ning, Habib, Sidra, Huang, Wei, Li, Zhengguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5526918/
https://www.ncbi.nlm.nih.gov/pubmed/28798762
http://dx.doi.org/10.3389/fpls.2017.01312
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author Yang, Lu
Hu, Guojian
Li, Ning
Habib, Sidra
Huang, Wei
Li, Zhengguo
author_facet Yang, Lu
Hu, Guojian
Li, Ning
Habib, Sidra
Huang, Wei
Li, Zhengguo
author_sort Yang, Lu
collection PubMed
description S-adenosylhomocysteine hydrolase (SAHH) functions as an enzyme catalyzing the reversible hydrolysis of S-adenosylhomocysteine to homocysteine and adenosine. In the present work we have investigated its role in the ripening process of tomato fruit. Among the three SlSAHH genes we demonstrated that SlSAHH2 was highly accumulated during fruit ripening and strongly responded to ethylene treatment. Over-expression of SlSAHH2 enhanced SAHH enzymatic activity in tomato fruit development and ripening stages and resulted in a major phenotypic change of reduced ripening time from anthesis to breaker. Consistent with this, the content of lycopene was higher in SlSAHH2 over-expression lines than in wild-type at the same developmental stage. The expression of two ethylene inducible genes (E4 and E8) and three ethylene biosynthesis genes (SlACO1, SlACO3 and SlACS2) increased to a higher level in SlSAHH2 over-expression lines at breaker stage, and one transgenic line even produced much more ethylene than wild-type. Although inconsistency in gene expression and ethylene production existed between the two transgenic lines, the transcriptional changes of several important ripening regulators such as RIN, AP2a, TAGL1, CNR and NOR showed a consistent pattern. It was speculated that the influence of SlSAHH2 on ethylene production was downstream of the regulation of SlSAHH2 on these ripening regulator genes. The over-expressing lines displayed higher sensitivity to ethylene in both fruit and non-fruit tissues. Ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) treatment accelerated ripening faster in SlSAHH2 over-expressing fruit than in wild-type. Additionally, seedlings of transgenic lines displayed shorter hypocotyls and roots in ethylene triple response assay. In conclusion, SlSAHH2 played an important role in tomato fruit ripening.
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spelling pubmed-55269182017-08-10 Functional Characterization of SlSAHH2 in Tomato Fruit Ripening Yang, Lu Hu, Guojian Li, Ning Habib, Sidra Huang, Wei Li, Zhengguo Front Plant Sci Plant Science S-adenosylhomocysteine hydrolase (SAHH) functions as an enzyme catalyzing the reversible hydrolysis of S-adenosylhomocysteine to homocysteine and adenosine. In the present work we have investigated its role in the ripening process of tomato fruit. Among the three SlSAHH genes we demonstrated that SlSAHH2 was highly accumulated during fruit ripening and strongly responded to ethylene treatment. Over-expression of SlSAHH2 enhanced SAHH enzymatic activity in tomato fruit development and ripening stages and resulted in a major phenotypic change of reduced ripening time from anthesis to breaker. Consistent with this, the content of lycopene was higher in SlSAHH2 over-expression lines than in wild-type at the same developmental stage. The expression of two ethylene inducible genes (E4 and E8) and three ethylene biosynthesis genes (SlACO1, SlACO3 and SlACS2) increased to a higher level in SlSAHH2 over-expression lines at breaker stage, and one transgenic line even produced much more ethylene than wild-type. Although inconsistency in gene expression and ethylene production existed between the two transgenic lines, the transcriptional changes of several important ripening regulators such as RIN, AP2a, TAGL1, CNR and NOR showed a consistent pattern. It was speculated that the influence of SlSAHH2 on ethylene production was downstream of the regulation of SlSAHH2 on these ripening regulator genes. The over-expressing lines displayed higher sensitivity to ethylene in both fruit and non-fruit tissues. Ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) treatment accelerated ripening faster in SlSAHH2 over-expressing fruit than in wild-type. Additionally, seedlings of transgenic lines displayed shorter hypocotyls and roots in ethylene triple response assay. In conclusion, SlSAHH2 played an important role in tomato fruit ripening. Frontiers Media S.A. 2017-07-26 /pmc/articles/PMC5526918/ /pubmed/28798762 http://dx.doi.org/10.3389/fpls.2017.01312 Text en Copyright © 2017 Yang, Hu, Li, Habib, Huang and Li. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Yang, Lu
Hu, Guojian
Li, Ning
Habib, Sidra
Huang, Wei
Li, Zhengguo
Functional Characterization of SlSAHH2 in Tomato Fruit Ripening
title Functional Characterization of SlSAHH2 in Tomato Fruit Ripening
title_full Functional Characterization of SlSAHH2 in Tomato Fruit Ripening
title_fullStr Functional Characterization of SlSAHH2 in Tomato Fruit Ripening
title_full_unstemmed Functional Characterization of SlSAHH2 in Tomato Fruit Ripening
title_short Functional Characterization of SlSAHH2 in Tomato Fruit Ripening
title_sort functional characterization of slsahh2 in tomato fruit ripening
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5526918/
https://www.ncbi.nlm.nih.gov/pubmed/28798762
http://dx.doi.org/10.3389/fpls.2017.01312
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