Transformation-induced changes in the DNA-nuclear matrix interface, revealed by high-throughput analysis of DNA halos

In higher eukaryotic nuclei, DNA is periodically anchored to an extraction-resistant protein structure, via matrix attachment regions. We describe a refined and accessible method to non-subjectively, rapidly and reproducibly measure both size and stability of the intervening chromatin loops, and use...

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Detalles Bibliográficos
Autores principales: Wilson, Rosemary H. C., Coverley, Dawn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5526987/
https://www.ncbi.nlm.nih.gov/pubmed/28743923
http://dx.doi.org/10.1038/s41598-017-06459-7
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author Wilson, Rosemary H. C.
Coverley, Dawn
author_facet Wilson, Rosemary H. C.
Coverley, Dawn
author_sort Wilson, Rosemary H. C.
collection PubMed
description In higher eukaryotic nuclei, DNA is periodically anchored to an extraction-resistant protein structure, via matrix attachment regions. We describe a refined and accessible method to non-subjectively, rapidly and reproducibly measure both size and stability of the intervening chromatin loops, and use it to demonstrate that malignant transformation compromises the DNA-nuclear matrix interface.
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spelling pubmed-55269872017-08-02 Transformation-induced changes in the DNA-nuclear matrix interface, revealed by high-throughput analysis of DNA halos Wilson, Rosemary H. C. Coverley, Dawn Sci Rep Article In higher eukaryotic nuclei, DNA is periodically anchored to an extraction-resistant protein structure, via matrix attachment regions. We describe a refined and accessible method to non-subjectively, rapidly and reproducibly measure both size and stability of the intervening chromatin loops, and use it to demonstrate that malignant transformation compromises the DNA-nuclear matrix interface. Nature Publishing Group UK 2017-07-25 /pmc/articles/PMC5526987/ /pubmed/28743923 http://dx.doi.org/10.1038/s41598-017-06459-7 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Wilson, Rosemary H. C.
Coverley, Dawn
Transformation-induced changes in the DNA-nuclear matrix interface, revealed by high-throughput analysis of DNA halos
title Transformation-induced changes in the DNA-nuclear matrix interface, revealed by high-throughput analysis of DNA halos
title_full Transformation-induced changes in the DNA-nuclear matrix interface, revealed by high-throughput analysis of DNA halos
title_fullStr Transformation-induced changes in the DNA-nuclear matrix interface, revealed by high-throughput analysis of DNA halos
title_full_unstemmed Transformation-induced changes in the DNA-nuclear matrix interface, revealed by high-throughput analysis of DNA halos
title_short Transformation-induced changes in the DNA-nuclear matrix interface, revealed by high-throughput analysis of DNA halos
title_sort transformation-induced changes in the dna-nuclear matrix interface, revealed by high-throughput analysis of dna halos
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5526987/
https://www.ncbi.nlm.nih.gov/pubmed/28743923
http://dx.doi.org/10.1038/s41598-017-06459-7
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