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Compounds that enhance the tailing activity of Moloney murine leukemia virus reverse transcriptase

In a previous study, we showed that MMLV-RT has a strong terminal transferase activity, and that the C-, G-, and T-tailing activities are enhanced by dGMP, dCMP, and dAMP, respectively. In this study, to achieve faster reaction and higher tailing efficiency, we screened other compounds for the abili...

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Detalles Bibliográficos
Autores principales: Ohtsubo, Yoshiyuki, Nagata, Yuji, Tsuda, Masataka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5529421/
https://www.ncbi.nlm.nih.gov/pubmed/28747695
http://dx.doi.org/10.1038/s41598-017-04765-8