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Porcine familial adenomatous polyposis model enables systematic analysis of early events in adenoma progression
We compared gene expression in low and high-grade intraepithelial dysplastic polyps from pigs carrying an APC (1311) truncating mutation orthologous to human APC (1309), analysing whole samples and microdissected dysplastic epithelium. Gene set enrichment analysis revealed differential expression of...
| Autores principales: | , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5529429/ https://www.ncbi.nlm.nih.gov/pubmed/28747659 http://dx.doi.org/10.1038/s41598-017-06741-8 |
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| author | Flisikowska, Tatiana Stachowiak, Monika Xu, Hongen Wagner, Alexandra Hernandez-Caceres, Alejandra Wurmser, Christine Perleberg, Carolin Pausch, Hubert Perkowska, Anna Fischer, Konrad Frishman, Dmitrij Fries, Ruedi Switonski, Marek Kind, Alexander Saur, Dieter Schnieke, Angelika Flisikowski, Krzysztof |
| author_facet | Flisikowska, Tatiana Stachowiak, Monika Xu, Hongen Wagner, Alexandra Hernandez-Caceres, Alejandra Wurmser, Christine Perleberg, Carolin Pausch, Hubert Perkowska, Anna Fischer, Konrad Frishman, Dmitrij Fries, Ruedi Switonski, Marek Kind, Alexander Saur, Dieter Schnieke, Angelika Flisikowski, Krzysztof |
| author_sort | Flisikowska, Tatiana |
| collection | PubMed |
| description | We compared gene expression in low and high-grade intraepithelial dysplastic polyps from pigs carrying an APC (1311) truncating mutation orthologous to human APC (1309), analysing whole samples and microdissected dysplastic epithelium. Gene set enrichment analysis revealed differential expression of gene sets similar to human normal mucosa versus T1 stage polyps. Transcriptome analysis of whole samples revealed many differentially-expressed genes reflecting immune infiltration. Analysis of microdissected dysplastic epithelium was markedly different and showed increased expression in high-grade intraepithelial neoplasia of several genes known to be involved in human CRC; and revealed possible new roles for GBP6 and PLXND1. The pig model thus facilitates analysis of CRC pathogenesis. |
| format | Online Article Text |
| id | pubmed-5529429 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2017 |
| publisher | Nature Publishing Group UK |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-55294292017-08-02 Porcine familial adenomatous polyposis model enables systematic analysis of early events in adenoma progression Flisikowska, Tatiana Stachowiak, Monika Xu, Hongen Wagner, Alexandra Hernandez-Caceres, Alejandra Wurmser, Christine Perleberg, Carolin Pausch, Hubert Perkowska, Anna Fischer, Konrad Frishman, Dmitrij Fries, Ruedi Switonski, Marek Kind, Alexander Saur, Dieter Schnieke, Angelika Flisikowski, Krzysztof Sci Rep Article We compared gene expression in low and high-grade intraepithelial dysplastic polyps from pigs carrying an APC (1311) truncating mutation orthologous to human APC (1309), analysing whole samples and microdissected dysplastic epithelium. Gene set enrichment analysis revealed differential expression of gene sets similar to human normal mucosa versus T1 stage polyps. Transcriptome analysis of whole samples revealed many differentially-expressed genes reflecting immune infiltration. Analysis of microdissected dysplastic epithelium was markedly different and showed increased expression in high-grade intraepithelial neoplasia of several genes known to be involved in human CRC; and revealed possible new roles for GBP6 and PLXND1. The pig model thus facilitates analysis of CRC pathogenesis. Nature Publishing Group UK 2017-07-26 /pmc/articles/PMC5529429/ /pubmed/28747659 http://dx.doi.org/10.1038/s41598-017-06741-8 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| spellingShingle | Article Flisikowska, Tatiana Stachowiak, Monika Xu, Hongen Wagner, Alexandra Hernandez-Caceres, Alejandra Wurmser, Christine Perleberg, Carolin Pausch, Hubert Perkowska, Anna Fischer, Konrad Frishman, Dmitrij Fries, Ruedi Switonski, Marek Kind, Alexander Saur, Dieter Schnieke, Angelika Flisikowski, Krzysztof Porcine familial adenomatous polyposis model enables systematic analysis of early events in adenoma progression |
| title | Porcine familial adenomatous polyposis model enables systematic analysis of early events in adenoma progression |
| title_full | Porcine familial adenomatous polyposis model enables systematic analysis of early events in adenoma progression |
| title_fullStr | Porcine familial adenomatous polyposis model enables systematic analysis of early events in adenoma progression |
| title_full_unstemmed | Porcine familial adenomatous polyposis model enables systematic analysis of early events in adenoma progression |
| title_short | Porcine familial adenomatous polyposis model enables systematic analysis of early events in adenoma progression |
| title_sort | porcine familial adenomatous polyposis model enables systematic analysis of early events in adenoma progression |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5529429/ https://www.ncbi.nlm.nih.gov/pubmed/28747659 http://dx.doi.org/10.1038/s41598-017-06741-8 |
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