One-step purification and immobilization of extracellularly expressed sortase A by magnetic particles to develop a robust and recyclable biocatalyst

Sortase A (SrtA) is a transpeptidase widely used to site-specifically modify peptides and proteins and shows promise for industrial applications. In this study, a novel strategy was developed for constructing immobilized-SrtA as a robust and recyclable enzyme via direct immobilization of extracellul...

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Detalles Bibliográficos
Autores principales: Zhao, Xinrui, Hong, Haofei, Cheng, Xiaozhong, Liu, Shaozhong, Deng, Tao, Guo, Zhongwu, Wu, Zhimeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5529518/
https://www.ncbi.nlm.nih.gov/pubmed/28747746
http://dx.doi.org/10.1038/s41598-017-06856-y

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5529518/
https://www.ncbi.nlm.nih.gov/pubmed/28747746
http://dx.doi.org/10.1038/s41598-017-06856-y

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