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Investigating asymmetric salt profiles for nanopore DNA sequencing with biological porin MspA

Nanopore DNA sequencing is a promising single-molecule analysis technology. This technique relies on a DNA motor enzyme to control movement of DNA precisely through a nanopore. Specific experimental buffer conditions are required based on the preferred operating conditions of the DNA motor enzyme. W...

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Detalles Bibliográficos
Autores principales: Nova, Ian C., Derrington, Ian M., Craig, Jonathan M., Noakes, Matthew T., Tickman, Benjamin I., Doering, Kenji, Higinbotham, Hugh, Laszlo, Andrew H., Gundlach, Jens H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5531483/
https://www.ncbi.nlm.nih.gov/pubmed/28749972
http://dx.doi.org/10.1371/journal.pone.0181599