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A modified FASP protocol for high-throughput preparation of protein samples for mass spectrometry

To facilitate high-throughput proteomic analyses we have developed a modified FASP protocol which improves the rate at which protein samples can be processed prior to mass spectrometry. Adapting the original FASP protocol to a 96-well format necessitates extended spin times for buffer exchange due t...

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Detalles Bibliográficos
Autores principales: Potriquet, Jeremy, Laohaviroj, Marut, Bethony, Jeffrey M., Mulvenna, Jason
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5531558/
https://www.ncbi.nlm.nih.gov/pubmed/28750034
http://dx.doi.org/10.1371/journal.pone.0175967