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High-resolution mapping of heteroduplex DNA formed during UV-induced and spontaneous mitotic recombination events in yeast

In yeast, DNA breaks are usually repaired by homologous recombination (HR). An early step for HR pathways is formation of a heteroduplex, in which a single-strand from the broken DNA molecule pairs with a strand derived from an intact DNA molecule. If the two strands of DNA are not identical, there...

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Autores principales: Yin, Yi, Dominska, Margaret, Yim, Eunice, Petes, Thomas D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5531827/
https://www.ncbi.nlm.nih.gov/pubmed/28714850
http://dx.doi.org/10.7554/eLife.28069
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author Yin, Yi
Dominska, Margaret
Yim, Eunice
Petes, Thomas D
author_facet Yin, Yi
Dominska, Margaret
Yim, Eunice
Petes, Thomas D
author_sort Yin, Yi
collection PubMed
description In yeast, DNA breaks are usually repaired by homologous recombination (HR). An early step for HR pathways is formation of a heteroduplex, in which a single-strand from the broken DNA molecule pairs with a strand derived from an intact DNA molecule. If the two strands of DNA are not identical, there will be mismatches within the heteroduplex DNA (hetDNA). In wild-type strains, these mismatches are repaired by the mismatch repair (MMR) system, producing a gene conversion event. In strains lacking MMR, the mismatches persist. Most previous studies involving hetDNA formed during mitotic recombination were restricted to one locus. Below, we present a global mapping of hetDNA formed in the MMR-defective mlh1 strain. We find that many recombination events are associated with repair of double-stranded DNA gaps and/or involve Mlh1-independent mismatch repair. Many of our events are not explicable by the simplest form of the double-strand break repair model of recombination. DOI: http://dx.doi.org/10.7554/eLife.28069.001
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spelling pubmed-55318272017-07-31 High-resolution mapping of heteroduplex DNA formed during UV-induced and spontaneous mitotic recombination events in yeast Yin, Yi Dominska, Margaret Yim, Eunice Petes, Thomas D eLife Genes and Chromosomes In yeast, DNA breaks are usually repaired by homologous recombination (HR). An early step for HR pathways is formation of a heteroduplex, in which a single-strand from the broken DNA molecule pairs with a strand derived from an intact DNA molecule. If the two strands of DNA are not identical, there will be mismatches within the heteroduplex DNA (hetDNA). In wild-type strains, these mismatches are repaired by the mismatch repair (MMR) system, producing a gene conversion event. In strains lacking MMR, the mismatches persist. Most previous studies involving hetDNA formed during mitotic recombination were restricted to one locus. Below, we present a global mapping of hetDNA formed in the MMR-defective mlh1 strain. We find that many recombination events are associated with repair of double-stranded DNA gaps and/or involve Mlh1-independent mismatch repair. Many of our events are not explicable by the simplest form of the double-strand break repair model of recombination. DOI: http://dx.doi.org/10.7554/eLife.28069.001 eLife Sciences Publications, Ltd 2017-07-17 /pmc/articles/PMC5531827/ /pubmed/28714850 http://dx.doi.org/10.7554/eLife.28069 Text en © 2017, Yin et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Genes and Chromosomes
Yin, Yi
Dominska, Margaret
Yim, Eunice
Petes, Thomas D
High-resolution mapping of heteroduplex DNA formed during UV-induced and spontaneous mitotic recombination events in yeast
title High-resolution mapping of heteroduplex DNA formed during UV-induced and spontaneous mitotic recombination events in yeast
title_full High-resolution mapping of heteroduplex DNA formed during UV-induced and spontaneous mitotic recombination events in yeast
title_fullStr High-resolution mapping of heteroduplex DNA formed during UV-induced and spontaneous mitotic recombination events in yeast
title_full_unstemmed High-resolution mapping of heteroduplex DNA formed during UV-induced and spontaneous mitotic recombination events in yeast
title_short High-resolution mapping of heteroduplex DNA formed during UV-induced and spontaneous mitotic recombination events in yeast
title_sort high-resolution mapping of heteroduplex dna formed during uv-induced and spontaneous mitotic recombination events in yeast
topic Genes and Chromosomes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5531827/
https://www.ncbi.nlm.nih.gov/pubmed/28714850
http://dx.doi.org/10.7554/eLife.28069
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