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Translational roles of the C75 2′OH in an in vitro tRNA transcript at the ribosomal A, P and E sites
Aminoacyl-tRNAs containing a deoxy substitution in the penultimate nucleotide (C75 2′OH → 2′H) have been widely used in translation for incorporation of unnatural amino acids (AAs). However, this supposedly innocuous modification surprisingly increased peptidyl-tRNA(Ala) (ugc) drop off in biochemica...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Nature Publishing Group UK
2017
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5532260/ https://www.ncbi.nlm.nih.gov/pubmed/28751745 http://dx.doi.org/10.1038/s41598-017-06991-6 |