Cargando…

ShadowY: a dark yellow fluorescent protein for FLIM-based FRET measurement

Fluorescence lifetime imaging microscopy (FLIM)-based Förster resonance energy transfer (FRET) measurement (FLIM-FRET) is one of the powerful methods for imaging of intracellular protein activities such as protein–protein interactions and conformational changes. Here, using saturation mutagenesis, w...

Descripción completa

Detalles Bibliográficos
Autores principales: Murakoshi, Hideji, Shibata, Akihiro C. E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5533704/
https://www.ncbi.nlm.nih.gov/pubmed/28754922
http://dx.doi.org/10.1038/s41598-017-07002-4