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Retrofitting the BAC cloning vector pBeloBAC11 by the insertion of a mutant loxP site
OBJECTIVE: Human genomic libraries constructed in bacterial artificial chromosome vectors were utilized to make physical maps of all 23-chromosome pairs and as the templates for DNA sequencing to aid in the completion of the Human Genome Project. The goal of this study was to modify the BAC vector p...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2017
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5534112/ https://www.ncbi.nlm.nih.gov/pubmed/28754135 http://dx.doi.org/10.1186/s13104-017-2631-8 |