A Simple and Cost-Effective Approach for In Vitro Production of Sliced siRNAs as Potent Triggers for RNAi

We have studied the molecular properties of in-vitro-transcribed sliced small interfering RNAs (tsli-siRNAs) as an alternative RNAi agent for chemically synthesized siRNA. We describe here a simple and cost-effective procedure for high-purity production of tsli-siRNA using bacteriophage T7 RNA polym...

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Detalles Bibliográficos
Autores principales: Sun, Guihua, Riggs, Arthur D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2017
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5537206/
https://www.ncbi.nlm.nih.gov/pubmed/28918034
http://dx.doi.org/10.1016/j.omtn.2017.07.008

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5537206/
https://www.ncbi.nlm.nih.gov/pubmed/28918034
http://dx.doi.org/10.1016/j.omtn.2017.07.008

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