Cargando…

Enriching Glucoraphanin in Brassica rapa Through Replacement of BrAOP2.2/BrAOP2.3 with Non-functional Genes

Sulforaphane, the hydrolytic product of glucoraphanin glucosinolate, is a potent anticarcinogen that reduces the risk of several human cancers. However, in most B. rapa vegetables, glucoraphanin is undetectable or only present in trace amounts, since the glucoraphanin that is present is converted to...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Zhiyuan, Liang, Jianli, Zheng, Shuning, Zhang, Jifang, Wu, Jian, Cheng, Feng, Yang, Wencai, Wang, Xiaowu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5539120/
https://www.ncbi.nlm.nih.gov/pubmed/28824667
http://dx.doi.org/10.3389/fpls.2017.01329
_version_ 1783254421801533440
author Liu, Zhiyuan
Liang, Jianli
Zheng, Shuning
Zhang, Jifang
Wu, Jian
Cheng, Feng
Yang, Wencai
Wang, Xiaowu
author_facet Liu, Zhiyuan
Liang, Jianli
Zheng, Shuning
Zhang, Jifang
Wu, Jian
Cheng, Feng
Yang, Wencai
Wang, Xiaowu
author_sort Liu, Zhiyuan
collection PubMed
description Sulforaphane, the hydrolytic product of glucoraphanin glucosinolate, is a potent anticarcinogen that reduces the risk of several human cancers. However, in most B. rapa vegetables, glucoraphanin is undetectable or only present in trace amounts, since the glucoraphanin that is present is converted to gluconapin by three functional BrAOP2 genes. In this study, to enrich beneficial glucoraphanin content in B. rapa, the functional BrAOP2 alleles were replaced by non-functional counterparts through marker-assisted backcrossing (MAB). We identified non-functional mutations of two BrAOP2 genes from B. rapa. The backcross progenies with introgression of both non-functional braop2.2 and braop2.3 alleles significantly increased the glucoraphanin content by 18 times relative to the recurrent parent. In contrast, replacement or introgression of single non-functional braop2.2 or braop2.3 locus did not change glucoraphanin content. Our results suggest that replacement of these two functional BrAOP2 genes with non-functional alleles has the potential for producing improved Brassica crops with enriched beneficial glucoraphanin content.
format Online
Article
Text
id pubmed-5539120
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-55391202017-08-18 Enriching Glucoraphanin in Brassica rapa Through Replacement of BrAOP2.2/BrAOP2.3 with Non-functional Genes Liu, Zhiyuan Liang, Jianli Zheng, Shuning Zhang, Jifang Wu, Jian Cheng, Feng Yang, Wencai Wang, Xiaowu Front Plant Sci Plant Science Sulforaphane, the hydrolytic product of glucoraphanin glucosinolate, is a potent anticarcinogen that reduces the risk of several human cancers. However, in most B. rapa vegetables, glucoraphanin is undetectable or only present in trace amounts, since the glucoraphanin that is present is converted to gluconapin by three functional BrAOP2 genes. In this study, to enrich beneficial glucoraphanin content in B. rapa, the functional BrAOP2 alleles were replaced by non-functional counterparts through marker-assisted backcrossing (MAB). We identified non-functional mutations of two BrAOP2 genes from B. rapa. The backcross progenies with introgression of both non-functional braop2.2 and braop2.3 alleles significantly increased the glucoraphanin content by 18 times relative to the recurrent parent. In contrast, replacement or introgression of single non-functional braop2.2 or braop2.3 locus did not change glucoraphanin content. Our results suggest that replacement of these two functional BrAOP2 genes with non-functional alleles has the potential for producing improved Brassica crops with enriched beneficial glucoraphanin content. Frontiers Media S.A. 2017-08-02 /pmc/articles/PMC5539120/ /pubmed/28824667 http://dx.doi.org/10.3389/fpls.2017.01329 Text en Copyright © 2017 Liu, Liang, Zheng, Zhang, Wu, Cheng, Yang and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Liu, Zhiyuan
Liang, Jianli
Zheng, Shuning
Zhang, Jifang
Wu, Jian
Cheng, Feng
Yang, Wencai
Wang, Xiaowu
Enriching Glucoraphanin in Brassica rapa Through Replacement of BrAOP2.2/BrAOP2.3 with Non-functional Genes
title Enriching Glucoraphanin in Brassica rapa Through Replacement of BrAOP2.2/BrAOP2.3 with Non-functional Genes
title_full Enriching Glucoraphanin in Brassica rapa Through Replacement of BrAOP2.2/BrAOP2.3 with Non-functional Genes
title_fullStr Enriching Glucoraphanin in Brassica rapa Through Replacement of BrAOP2.2/BrAOP2.3 with Non-functional Genes
title_full_unstemmed Enriching Glucoraphanin in Brassica rapa Through Replacement of BrAOP2.2/BrAOP2.3 with Non-functional Genes
title_short Enriching Glucoraphanin in Brassica rapa Through Replacement of BrAOP2.2/BrAOP2.3 with Non-functional Genes
title_sort enriching glucoraphanin in brassica rapa through replacement of braop2.2/braop2.3 with non-functional genes
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5539120/
https://www.ncbi.nlm.nih.gov/pubmed/28824667
http://dx.doi.org/10.3389/fpls.2017.01329
work_keys_str_mv AT liuzhiyuan enrichingglucoraphanininbrassicarapathroughreplacementofbraop22braop23withnonfunctionalgenes
AT liangjianli enrichingglucoraphanininbrassicarapathroughreplacementofbraop22braop23withnonfunctionalgenes
AT zhengshuning enrichingglucoraphanininbrassicarapathroughreplacementofbraop22braop23withnonfunctionalgenes
AT zhangjifang enrichingglucoraphanininbrassicarapathroughreplacementofbraop22braop23withnonfunctionalgenes
AT wujian enrichingglucoraphanininbrassicarapathroughreplacementofbraop22braop23withnonfunctionalgenes
AT chengfeng enrichingglucoraphanininbrassicarapathroughreplacementofbraop22braop23withnonfunctionalgenes
AT yangwencai enrichingglucoraphanininbrassicarapathroughreplacementofbraop22braop23withnonfunctionalgenes
AT wangxiaowu enrichingglucoraphanininbrassicarapathroughreplacementofbraop22braop23withnonfunctionalgenes