A versatile one-step CRISPR-Cas9 based approach to plasmid-curing

BACKGROUND: Plasmids are widely used and essential tools in molecular biology. However, plasmids often impose a metabolic burden and are only temporarily useful for genetic engineering, bio-sensing and characterization purposes. While numerous techniques for genetic manipulation exist, a universal t...

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Detalles Bibliográficos
Autores principales: Lauritsen, Ida, Porse, Andreas, Sommer, Morten O. A., Nørholm, Morten H. H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Technical Notes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5540278/
https://www.ncbi.nlm.nih.gov/pubmed/28764701
http://dx.doi.org/10.1186/s12934-017-0748-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5540278/
https://www.ncbi.nlm.nih.gov/pubmed/28764701
http://dx.doi.org/10.1186/s12934-017-0748-z

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