Immunocytochemistry and fluorescence imaging efficiently identify individual neurons with CRISPR/Cas9-mediated gene disruption in primary cortical cultures

BACKGROUND: CRISPR/Cas9 system is a powerful method to investigate the role of genes by introducing a mutation selectively and efficiently to specific genome positions in cell and animal lines. However, in primary neuron cultures, this method is affected by the issue that the effectiveness of CRISPR...

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Detalles Bibliográficos
Autores principales: Tsunematsu, Hiroto, Uyeda, Akiko, Yamamoto, Nobuhiko, Sugo, Noriyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5540436/
https://www.ncbi.nlm.nih.gov/pubmed/28764650
http://dx.doi.org/10.1186/s12868-017-0377-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5540436/
https://www.ncbi.nlm.nih.gov/pubmed/28764650
http://dx.doi.org/10.1186/s12868-017-0377-9

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