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Three-dimensional (3D) evaluation of liquid distribution in shake flask using an optical fluorescence technique
BACKGROUND: Biotechnological development in shake flask necessitates vital engineering parameters e.g. volumetric power input, mixing time, gas liquid mass transfer coefficient, hydromechanical stress and effective shear rate. Determination and optimization of these parameters through experiments ar...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5541408/ https://www.ncbi.nlm.nih.gov/pubmed/28785308 http://dx.doi.org/10.1186/s13036-017-0070-7 |
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author | Azizan, Amizon Büchs, Jochen |
author_facet | Azizan, Amizon Büchs, Jochen |
author_sort | Azizan, Amizon |
collection | PubMed |
description | BACKGROUND: Biotechnological development in shake flask necessitates vital engineering parameters e.g. volumetric power input, mixing time, gas liquid mass transfer coefficient, hydromechanical stress and effective shear rate. Determination and optimization of these parameters through experiments are labor-intensive and time-consuming. Computational Fluid Dynamics (CFD) provides the ability to predict and validate these parameters in bioprocess engineering. This work provides ample experimental data which are easily accessible for future validations to represent the hydrodynamics of the fluid flow in the shake flask. RESULTS: A non-invasive measuring technique using an optical fluorescence method was developed for shake flasks containing a fluorescent solution with a waterlike viscosity at varying filling volume (V(L) = 15 to 40 mL) and shaking frequency (n = 150 to 450 rpm) at a constant shaking diameter (d(o) = 25 mm). The method detected the leading edge (LB) and tail of the rotating bulk liquid (TB) relative to the direction of the centrifugal acceleration at varying circumferential heights from the base of the shake flask. The determined LB and TB points were translated into three-dimensional (3D) circumferential liquid distribution plots. The maximum liquid height (H(max)) of the bulk liquid increased with increasing filling volume and shaking frequency of the shaking flask, as expected. The toroidal shapes of LB and TB are clearly asymmetrical and the measured TB differed by the elongation of the liquid particularly towards the torus part of the shake flask. CONCLUSION: The 3D liquid distribution data collected at varying filling volume and shaking frequency, comprising of LB and TB values relative to the direction of the centrifugal acceleration are essential for validating future numerical solutions using CFD to predict vital engineering parameters in shake flask. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13036-017-0070-7) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5541408 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-55414082017-08-07 Three-dimensional (3D) evaluation of liquid distribution in shake flask using an optical fluorescence technique Azizan, Amizon Büchs, Jochen J Biol Eng Research BACKGROUND: Biotechnological development in shake flask necessitates vital engineering parameters e.g. volumetric power input, mixing time, gas liquid mass transfer coefficient, hydromechanical stress and effective shear rate. Determination and optimization of these parameters through experiments are labor-intensive and time-consuming. Computational Fluid Dynamics (CFD) provides the ability to predict and validate these parameters in bioprocess engineering. This work provides ample experimental data which are easily accessible for future validations to represent the hydrodynamics of the fluid flow in the shake flask. RESULTS: A non-invasive measuring technique using an optical fluorescence method was developed for shake flasks containing a fluorescent solution with a waterlike viscosity at varying filling volume (V(L) = 15 to 40 mL) and shaking frequency (n = 150 to 450 rpm) at a constant shaking diameter (d(o) = 25 mm). The method detected the leading edge (LB) and tail of the rotating bulk liquid (TB) relative to the direction of the centrifugal acceleration at varying circumferential heights from the base of the shake flask. The determined LB and TB points were translated into three-dimensional (3D) circumferential liquid distribution plots. The maximum liquid height (H(max)) of the bulk liquid increased with increasing filling volume and shaking frequency of the shaking flask, as expected. The toroidal shapes of LB and TB are clearly asymmetrical and the measured TB differed by the elongation of the liquid particularly towards the torus part of the shake flask. CONCLUSION: The 3D liquid distribution data collected at varying filling volume and shaking frequency, comprising of LB and TB values relative to the direction of the centrifugal acceleration are essential for validating future numerical solutions using CFD to predict vital engineering parameters in shake flask. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13036-017-0070-7) contains supplementary material, which is available to authorized users. BioMed Central 2017-08-03 /pmc/articles/PMC5541408/ /pubmed/28785308 http://dx.doi.org/10.1186/s13036-017-0070-7 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Azizan, Amizon Büchs, Jochen Three-dimensional (3D) evaluation of liquid distribution in shake flask using an optical fluorescence technique |
title | Three-dimensional (3D) evaluation of liquid distribution in shake flask using an optical fluorescence technique |
title_full | Three-dimensional (3D) evaluation of liquid distribution in shake flask using an optical fluorescence technique |
title_fullStr | Three-dimensional (3D) evaluation of liquid distribution in shake flask using an optical fluorescence technique |
title_full_unstemmed | Three-dimensional (3D) evaluation of liquid distribution in shake flask using an optical fluorescence technique |
title_short | Three-dimensional (3D) evaluation of liquid distribution in shake flask using an optical fluorescence technique |
title_sort | three-dimensional (3d) evaluation of liquid distribution in shake flask using an optical fluorescence technique |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5541408/ https://www.ncbi.nlm.nih.gov/pubmed/28785308 http://dx.doi.org/10.1186/s13036-017-0070-7 |
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