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Binding of ZO-1 to α5β1 integrins regulates the mechanical properties of α5β1–fibronectin links

Fundamental processes in cell adhesion, motility, and rigidity adaptation are regulated by integrin-mediated adhesion to the extracellular matrix (ECM). The link between the ECM component fibronectin (fn) and integrin α5β1 forms a complex with ZO-1 in cells at the edge of migrating monolayers, regul...

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Autores principales: González-Tarragó, Víctor, Elosegui-Artola, Alberto, Bazellières, Elsa, Oria, Roger, Pérez-González, Carlos, Roca-Cusachs, Pere
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5541835/
https://www.ncbi.nlm.nih.gov/pubmed/28251923
http://dx.doi.org/10.1091/mbc.E17-01-0006
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author González-Tarragó, Víctor
Elosegui-Artola, Alberto
Bazellières, Elsa
Oria, Roger
Pérez-González, Carlos
Roca-Cusachs, Pere
author_facet González-Tarragó, Víctor
Elosegui-Artola, Alberto
Bazellières, Elsa
Oria, Roger
Pérez-González, Carlos
Roca-Cusachs, Pere
author_sort González-Tarragó, Víctor
collection PubMed
description Fundamental processes in cell adhesion, motility, and rigidity adaptation are regulated by integrin-mediated adhesion to the extracellular matrix (ECM). The link between the ECM component fibronectin (fn) and integrin α5β1 forms a complex with ZO-1 in cells at the edge of migrating monolayers, regulating cell migration. However, how this complex affects the α5β1-fn link is unknown. Here we show that the α5β1/ZO-1 complex decreases the resistance to force of α5β1–fn adhesions located at the edge of migrating cell monolayers while also increasing α5β1 recruitment. Consistently with a molecular clutch model of adhesion, this effect of ZO-1 leads to a decrease in the density and intensity of adhesions in cells at the edge of migrating monolayers. Taken together, our results unveil a new mode of integrin regulation through modification of the mechanical properties of integrin–ECM links, which may be harnessed by cells to control adhesion and migration.
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spelling pubmed-55418352017-09-22 Binding of ZO-1 to α5β1 integrins regulates the mechanical properties of α5β1–fibronectin links González-Tarragó, Víctor Elosegui-Artola, Alberto Bazellières, Elsa Oria, Roger Pérez-González, Carlos Roca-Cusachs, Pere Mol Biol Cell Brief Reports Fundamental processes in cell adhesion, motility, and rigidity adaptation are regulated by integrin-mediated adhesion to the extracellular matrix (ECM). The link between the ECM component fibronectin (fn) and integrin α5β1 forms a complex with ZO-1 in cells at the edge of migrating monolayers, regulating cell migration. However, how this complex affects the α5β1-fn link is unknown. Here we show that the α5β1/ZO-1 complex decreases the resistance to force of α5β1–fn adhesions located at the edge of migrating cell monolayers while also increasing α5β1 recruitment. Consistently with a molecular clutch model of adhesion, this effect of ZO-1 leads to a decrease in the density and intensity of adhesions in cells at the edge of migrating monolayers. Taken together, our results unveil a new mode of integrin regulation through modification of the mechanical properties of integrin–ECM links, which may be harnessed by cells to control adhesion and migration. The American Society for Cell Biology 2017-07-07 /pmc/articles/PMC5541835/ /pubmed/28251923 http://dx.doi.org/10.1091/mbc.E17-01-0006 Text en © 2017 González-Tarragó et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Brief Reports
González-Tarragó, Víctor
Elosegui-Artola, Alberto
Bazellières, Elsa
Oria, Roger
Pérez-González, Carlos
Roca-Cusachs, Pere
Binding of ZO-1 to α5β1 integrins regulates the mechanical properties of α5β1–fibronectin links
title Binding of ZO-1 to α5β1 integrins regulates the mechanical properties of α5β1–fibronectin links
title_full Binding of ZO-1 to α5β1 integrins regulates the mechanical properties of α5β1–fibronectin links
title_fullStr Binding of ZO-1 to α5β1 integrins regulates the mechanical properties of α5β1–fibronectin links
title_full_unstemmed Binding of ZO-1 to α5β1 integrins regulates the mechanical properties of α5β1–fibronectin links
title_short Binding of ZO-1 to α5β1 integrins regulates the mechanical properties of α5β1–fibronectin links
title_sort binding of zo-1 to α5β1 integrins regulates the mechanical properties of α5β1–fibronectin links
topic Brief Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5541835/
https://www.ncbi.nlm.nih.gov/pubmed/28251923
http://dx.doi.org/10.1091/mbc.E17-01-0006
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