An efficient method to enrich for knock-out and knock-in cellular clones using the CRISPR/Cas9 system

Clustered Regularly Interspaced Short Palindromic Repeats-associated protein 9 nuclease (CRISPR/Cas9) and Transcription Activator-Like Effector Nucleases (TALENs) are versatile tools for genome editing. Here we report a method to increase the frequency of Cas9-targeted cellular clones. Our method is...

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Detalles Bibliográficos
Autores principales: Niccheri, Francesca, Pecori, Riccardo, Conticello, Silvestro G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2017
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5544813/
https://www.ncbi.nlm.nih.gov/pubmed/28421278
http://dx.doi.org/10.1007/s00018-017-2524-y

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5544813/
https://www.ncbi.nlm.nih.gov/pubmed/28421278
http://dx.doi.org/10.1007/s00018-017-2524-y

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