Polymer-encased nanodiscs with improved buffer compatibility

Styrene-maleic acid copolymers allow for solubilization and reconstitution of membrane proteins into nanodiscs. These polymer-encased nanodiscs are promising platforms for studies of membrane proteins in a near-physiologic environment without the use of detergents. However, current styrene-maleic ac...

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Autores principales: Fiori, Mariana C., Jiang, Yunjiang, Altenberg, Guillermo A., Liang, Hongjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5547149/
https://www.ncbi.nlm.nih.gov/pubmed/28785023
http://dx.doi.org/10.1038/s41598-017-07110-1
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author Fiori, Mariana C.
Jiang, Yunjiang
Altenberg, Guillermo A.
Liang, Hongjun
author_facet Fiori, Mariana C.
Jiang, Yunjiang
Altenberg, Guillermo A.
Liang, Hongjun
author_sort Fiori, Mariana C.
collection PubMed
description Styrene-maleic acid copolymers allow for solubilization and reconstitution of membrane proteins into nanodiscs. These polymer-encased nanodiscs are promising platforms for studies of membrane proteins in a near-physiologic environment without the use of detergents. However, current styrene-maleic acid copolymers display severe limitations in terms of buffer compatibility and ensued flexibility for various applications. Here, we present a new family of styrene-maleic acid copolymers that do not aggregate at low pH or in the presence of polyvalent cations, and can be used to solubilize membrane proteins and produce nanodiscs of controlled sizes.
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spelling pubmed-55471492017-08-09 Polymer-encased nanodiscs with improved buffer compatibility Fiori, Mariana C. Jiang, Yunjiang Altenberg, Guillermo A. Liang, Hongjun Sci Rep Article Styrene-maleic acid copolymers allow for solubilization and reconstitution of membrane proteins into nanodiscs. These polymer-encased nanodiscs are promising platforms for studies of membrane proteins in a near-physiologic environment without the use of detergents. However, current styrene-maleic acid copolymers display severe limitations in terms of buffer compatibility and ensued flexibility for various applications. Here, we present a new family of styrene-maleic acid copolymers that do not aggregate at low pH or in the presence of polyvalent cations, and can be used to solubilize membrane proteins and produce nanodiscs of controlled sizes. Nature Publishing Group UK 2017-08-07 /pmc/articles/PMC5547149/ /pubmed/28785023 http://dx.doi.org/10.1038/s41598-017-07110-1 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Fiori, Mariana C.
Jiang, Yunjiang
Altenberg, Guillermo A.
Liang, Hongjun
Polymer-encased nanodiscs with improved buffer compatibility
title Polymer-encased nanodiscs with improved buffer compatibility
title_full Polymer-encased nanodiscs with improved buffer compatibility
title_fullStr Polymer-encased nanodiscs with improved buffer compatibility
title_full_unstemmed Polymer-encased nanodiscs with improved buffer compatibility
title_short Polymer-encased nanodiscs with improved buffer compatibility
title_sort polymer-encased nanodiscs with improved buffer compatibility
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5547149/
https://www.ncbi.nlm.nih.gov/pubmed/28785023
http://dx.doi.org/10.1038/s41598-017-07110-1
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