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Simultaneous detection of three pome fruit tree viruses by one-step multiplex quantitative RT-PCR

A one-step multiplex real-time reverse transcription polymerase chain reaction (RT-qPCR) based on TaqMan probes was developed for the simultaneous detection of Apple mosaic virus (ApMV), Apple stem pitting virus (ASPV) and Apple stem grooving virus (ASGV) in total RNA of pome trees extracted with a...

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Detalles Bibliográficos
Autores principales: Malandraki, Ioanna, Beris, Despoina, Isaioglou, Ioannis, Olmos, Antonio, Varveri, Christina, Vassilakos, Nikon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5547701/
https://www.ncbi.nlm.nih.gov/pubmed/28749955
http://dx.doi.org/10.1371/journal.pone.0180877